Method for isolating dithiochrome, an insulin-binding molecule w

Details Method for isolating dithiochrome, an insulin-binding molecule w Method for isolating dithiochrome, an insulin-binding molecule w

1991-06-11

1992-04-28

Silverman, Stanley S.

Liquid purification or separation

Processes

Liquid/liquid solvent or colloidal extraction or diffusing...

530304, 530305, 530413, 530417, 514866, 514504, 425655, 424655, B01D 1508

Patent

active

051086107

ABSTRACT:
A process for obtaining a material possessing glucose tolerance factor activity and capable of binding with insulin including the steps of (i) contacting a eukaryotic cell mass with a mixture of water and alcohol to form a water phase and an alcohol phase; (ii) separating the alcohol phase from the water phase and isolating a water phase extract; and (iii) subjecting the water phase extract to gel exclusion chromatography, eluting all material having a molecular weight of approximately 720 to 1120 to obtain a material possessing glucose tolerance factor activity and capable of binding with insulin.

REFERENCES:
patent: 4985439 (1991-01-01), Hwang et al.
"Preparation of Chromium-Containing Material of Glucose Tolerance Factor Activity from Brewer's Yeast Extracts and by Synthesis", J. Agric. Food Chem., vol. 25, No. 1, pp. 162-166, Toepfer et al., 1977.
"Present Knowledge of the Role of Chromium", Federation Proceedings, vol. 33, No. 11, pp. 2275-2280, Mertz et al., Nov. 1974.

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