Liquid purification or separation – Processes – Chromatography
Patent
1989-09-19
1991-10-22
Silverman, Stanley S.
Liquid purification or separation
Processes
Chromatography
530304, 530305, 530413, 530417, B01D 1508
Patent
active
050593293
ABSTRACT:
A process for purifying dithiochrome from a natural source is disclosed. The process comprises: (i) contacting a eukaryotic cell mass with a mixture of water and an alcohol; (ii) separating the alcohol phase from the water phase and obtaining a water phase extract; (iii) subjecting the water phase extract to a sulfhydryl exchange chromatography matrix; (iv) washing said chromatography matrix; and (v) treating said chromatrography matrix with an active thiol to obtain dithiochromate; a material capable of binding with insulin. Dithiochromate may be used to treat patients suffering from elevated blood glucose or suboptimal glucose kinetics. An adduct of this compound with insulin may be used to treat patients for type I diabetes.
REFERENCES:
patent: 4432877 (1984-02-01), Tzodikov
patent: 4629567 (1986-12-01), Bollen et al.
"Preparation of Chromium--Containing Material of Glucose Tolerance Factor Activity from Brewer's Yeast Extracts and by Synthesis", J. Agric. Food Chem., vol. 25, No. 1, pp. 162-166, Toepfer, et al., 1977.
Mertz et al, Federation Proceedings, vol. 33, No. 11 (Nov. 1974) pp. 2275-2280 "Present knowledge of the role of chromium".
McCarthy Neil M.
Silverman Stanley S.
Thymax Corporation
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