Method for isolating cells from tissue with a composition contai

Chemistry: molecular biology and microbiology – Process of utilizing an enzyme or micro-organism to destroy... – Treating animal or plant material or micro-organism

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4352402, 435243, 435267, 435219, C07G 1500, C12N 500, C12N 100, C12N 950

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active

054242088

ABSTRACT:
Proteolytic enzyme compositions and processes for digesting connective tissue are provided. The enzyme compositions include collagenase, which is essentially free of toxins and non-collagen specific components, and chymopapain, which is essentially free of toxins. The enzyme compositions are used for dissociating microvessel cells from connective tissue. Recovered microvessel cells are incorporated into artificial vessel grafts. The composition preferrably contains collagenase having an activity of about 43 nkat/ml to about 51 nkat/ml and chymopapin having an activity of about 0.22 nkat/ml to about 0.44 nkat/ml.

REFERENCES:
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patent: 5116615 (1992-05-01), Gokcen
Tavakol, K. et al. "Enhanced dissolution of nucleus pulposus: Combined enzyme approach" Surgical Forum (USA), 1986, vol. 37 pp. 491-494.

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