Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification
Patent
1991-05-31
1993-09-14
Russel, Jeffrey E.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Separation or purification
530350, 530395, 530415, 530828, C07K 302, C07K 320, C07K 1506, C07K 1514
Patent
active
052450171
ABSTRACT:
Disclosed are methods of isolating a protein which binds carcinoembryonic antigen. These methods include the following steps. A biological sample containing carcinoembryonic antigen (CEA) and a CEA-binding protein (CBP) is provided and contacted with a divalent cation at a concentration and for a time sufficient to allow the binding of the CBP to CEA, thereby forming a CBP-CEA conjugate. The sample is then contacted with an adsorbent that binds CEA for a time sufficient to allow adsorbance to the adsorbent. Portions of the sample not adsorbed are removed. The CBP is then disassociated from the conjugate and is collected.
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Briggman Joseph V.
Maswoswe Sibusisiwe M.
Thomas Peter
Toth Carol A.
Applied Biotechnology, Inc.
New England Deaconess Hospital Corporation
Russel Jeffrey E.
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