Chemistry: analytical and immunological testing – Including sample preparation – Liberation or purification of sample or separation of...
Patent
1997-03-03
1999-05-04
Ludlow, Jan
Chemistry: analytical and immunological testing
Including sample preparation
Liberation or purification of sample or separation of...
210787, 210789, 436 57, 436 63, 436174, 604 6, G01N 118, G01N 128
Patent
active
059003774
ABSTRACT:
Disclosed is a method for separating leukocytes from platelets. Blood serum is added to a centrifuge tube. A suspension containing leukocytes and platelets is then added to form a layer on top of the blood serum, the layer having a density less than the density of the blood serum. The cells and the layers are centrifuged at a force and for a time sufficient to separate the leukocytes from the platelets. The resulting, isolated leukocytes are then recovered from the centrifuge tube. In some embodiments, a suspension of the leukocyte cells are pretreated by incubating with a stannous ion reducing agent, the suspension having a stannous ion concentration of from about 50 .mu.g to about 1000 .mu.g stannous ion per 10.sup.8 leukocyte cells. A suspension of the pretreated leukocyte cells is then incubated with a solution containing a technetium-99m (VII) salt, preferably sodium .sup.99m Tc-pertechnetate, to radiolabel the leukocyte cells.
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Gotti Paul Richard
Rees Trenton Todd
Ludlow Jan
Syncor International Corporation
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