Method for isolating and purifying bordetella pertussis antigeni

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Glycoprotein – e.g. – mucins – proteoglycans – etc.

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530415, 530417, 530825, C07K 302, C07K 320, C07K 1504

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053917154

ABSTRACT:
A process is provided for the purification of pertussis toxin (PT) and/or filamentous hemagglutinin antigen (FHA) from a B. pertussis fermentation broth or cell free culture supernatant containing at least one antigen, which comprises contacting the fermenation broth or cell free culture supernatant with a hydroxyapatite adsorbent for a sufficient time to adsorb the antigen(s) at a pH which both PT and FHA are adsorbed and eluting a mixture containing the adsorbed antigen(s) from the adsorbent with eluant at a pH which both PT and FHA are eluted. The PT and FHA may be further purified by two sequential chromatographic columns, one of which involves apolar-ligand chromatography.

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patent: 4997915 (1991-03-01), Tan et al.
Y. Sato, et al., The Lancet, Jan. 21, 1984, pp. 122-126.
M. Christodoulides, et al., Vaccine, vol. 5, Sep. 1987, pp. 199-207.
M. Svoboda, et al., Analytical Biochemistry, 159:402-411 (1986).
R. D. Sekura, et al., J. Biol. Chem., 258:14647-14651 (1983).
Y. Sato, et al., Infection and Immunicty, vol. 41, No. 1, Jul. 1983, pp. 313-320.
M. Chazono, et al., J. Biol. Standardization, 16:83-89 (1988).
J. J. Munoz, et al., Infection and Immunity, vol. 32, No. 1, Apr. 1981, pp. 243-250.
Chem. Abstract 102(9):77281z (1985) (equivalent to JP 59175439A).
Fundamentals of Biotechnology, Prave et al, eds. VCH. 1987. p. 311.

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