Method for isolating and directly cloning genes which encode cel

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

4353201, 4351723, C12N 510, C12N 1585

Patent

active

056655908

ABSTRACT:
Methods and DNA cloning vectors are provided for obtaining genes encoding secreted proteins and cell surface proteins.

REFERENCES:
patent: 5525486 (1996-06-01), Honjo et al.
patent: 5536637 (1996-07-01), Jacobs
Tashiro et al., "Signal sequence trap: A cloning strategy for secreted proteins and type 1 membrane proteins" Science (1993) 261:600-603.
Sambrook et al., eds., Molecular Cloning. A Laboratory Manual (1989) 2nd Ed., vols. 1-3, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York. The title page and table of contents are included herewith.
Ausubel et al., eds., Current Protocols in Molecular Biology (1993) vol. 2, Current Protocols, New York. The title page and table of contents are included herewith.
Fujisawa et al., "A transcriptional enhancer sequence of HTLV-I is responsible for trans-activation mediated by p40.sup.x of HTLV-I" EMBO J. (1986) 5:713-718.
Ohtani et al., "Identification of two distinct elements in the long terminal repeat of HTLV-I responsible for maximum gene expression" EMBO J. (1987) 6:389-395.
Takebe et al., "SR.alpha. promoter: an efficient and versatile mammalian cDNA expression system composed of the simian virus 40 early promoter and the R-U5 segment of human T-cell leukemia virus type 1 long terminal repeat" Mol. Cell. Biol. (1988) 8:466-472.
Metzger et al., "The human oestrogen receptor functions in yeast" Nature (1988) 334:31-36.
Fiers et al., "Complete nucleotide sequence of SV40 DNA" Nature (1978) 273:113-120.
Gluzman et al., eds., Enhancers and Eukaryotic Gene Expression (1983) Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York. The title page and table of contents are included herewith.
Seed, "An LFA-3 cDNA encodes a phospholipid-linked membrane protein homologous to its receptor CD2" Nature (1987) 329:840-841.
Evan et al., "Isolation of monoclonal antibodies specific for human c-myc protooncogene product" Mol. Cell. Biol. (1985) 5:3610-3616.
Wilson et al., "The structure of an antigenic determinant in a protein" Cell (1984) 37:767-778.
Ferguson et al., "Cell-surface anchoring of proteins via glycosyl-phosphatidylinositol structures" Ann. Rev. Biochem. (1988) 57:285-320.
Beaucage et al., "Deoxynucleoside phosphoramidites--a new class of key intermediates for deoxypolynucleotide synthesis" Tetra. Letts. (1981) 22:1859-1862.
Matteucci et al, "Synthesis of deoxyoligonucleotides on a polymer support" J. Am. Chem. Soc. (1981) 103:3185-3191.
Hemperly et al., "Sequence of a cDNA clone encoding the polysialic acid-rich and cytoplasmic domains of the neural cell adhesion molecule N-CAM" Proc. Natl. Acad. Sci. USA (1986) 83:3037-3041.
Barthels et al., "Isolation and nucleotide sequence of mouse NCAM cDNA that codes for a M.sub.r 79 000 polypeptide without a membrane-spanning region" EMBO J. (1987) 6:907-914.
Barthels et al, "High degree of NCAM diversity generated by alternative RNA splicing in brain and muscle" Eur. J. Neurosci. (1992) 4:327-337.
Crossin et al., "Expression of adhesion molecules and the establishment of boundaries during embryonic and neural development" Exp. Neurol. (1990) 109:6-18.
Tosney et al., "The distribution of NCAM in the chick hindlimb during axon outgrowth and synaptogenesis" Dev. Biol. (1986) 114:437-452.
Thiery et al., "Adhesion among neural cells of the chick embryo: II. Purification and characterization of a cell adhesion molecule from neural retina" J. Biol. Chem. (1977) 252:6841-6845.
Key et al., "Olfactory neurons express a unique glycosylated form of the neural cell adhesion molecule (N-CAM)" J. Cell. Biol. (1990) 110:1729-1743.
Edelman, "Morphoregulatory molecules" Biochem. (1988) 27:3533-3543.
Kanehisa, "Use of statistical criteria for screening potential homologies in nucleic acid sequences" Nucl. Acids Res. (1984) 12:203-213.
Mullis et al., "Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction" Quant. Biol. (1986) 51:263-273.
Hanahan, "Studies on transformation of Escherichia coli with plasmids" J. Mol. Biol. (1983) 166:557-580.
Matthews et al., "A receptor tyrosine kinase specific to hematopoietic stem and progenitor cell-enriched populations" Cell (1991 ) 65:1143-1152.
Baum et al., "Isolation of a candidate human hematopoietic stem-cell population" Proc. Natl. Acad. Sci. USA (1992) 89:2804-2808.
Rosnet et al., "Human FLT3/FLK2 gene: cDNA cloning and expression in hematopoietic cells" Blood (1993) 82:1110-1119.
Anderson et al., "Molecular cloning of mast cell growth factor, a hematopoietin that is active in both membrane bound and soluble forms" Cell (1990) 63:235-243.
Chirgwin et al., "Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease" Biochem. (1979) 18:5294-5299.
Veres et al., "The molecular basis of the sparse fur mouse mutation" Science (1987) 237:415-417.
Gu et al., "Cell cycle regulation of CDK2 activity by phosphorylation of Thr160 and Tyr 15" EMBO J. (1992) 11:3995-4005.
Tsai et al., "Isolation of the human cdk2 gene that encodes the cyclin A- and adenovirus E1A-associated p33 kinase" Nature (1991) 353:174-177.
Kaufman, "Vectors used for expression in mammalian cells" Meth. Enzymol. (1990) 185:487-511.
Ferguson, "Cell-surface anchoring of proteins via glycosyl-phosphatidylinositol structures" Ann. Rev. Biochem. (1988) 57:285-320.
Sabatini et al, "Mechanisms for the incorporation of proteins in membranes and organelles" J. Cell. Biol. (1982) 92:1-22.
Wilson et al., "The structure of an antigenic determinant in a protein" Cell (1984) 37:767-778.
Hirt, "Selective extraction of polyoma DNA from infected mouse cell cultures" J. Mol. Biol. (1967) 26:365-369.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Method for isolating and directly cloning genes which encode cel does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for isolating and directly cloning genes which encode cel, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for isolating and directly cloning genes which encode cel will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-69191

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.