Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Reexamination Certificate
2006-09-26
2006-09-26
Sitton, Jehanne (Department: 1634)
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
C536S023700, C536S024300, C536S024320
Reexamination Certificate
active
07112663
ABSTRACT:
A method for isolating a polynucleotide of interest that is present in the genome of a first mycobacterium strain and/or is expressed by the first mycobacterium strain, where the polynucleotide of interest is also absent or altered in the genome of a second mycobacterium strain and/or is not expressed in the second mycobacterium. The method includes (a) contacting the genomic DNA of the first mycobacterium strain under hybridizing conditions with the DNA of a least one clone that belongs to a bacterial artificial chromosome (BAC) genomic DNA library of the second mycobacterium strain, and (b) isolating the polynucleotide of interest that does not form a hybrid with the DNA of the second mycobacterium strain. This invention further pertains to aMycobacterium tuberculosisstrain H37Rv genomic DNA library, as well as aMycobacterium bovisBCG strain Pasteur genomic DNA library, and the recombinant BAC vectors that belong to those genomic DNA libraries. This invention also relates to mycobacterial nucleic acids, and methods and kits for using these nucleic acids to detect mycobacteria in a biological sample.
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Genbank Accession No. AD000001; Dec. 1996.
Blast alignment between Seq ID Nos. 1 and GI 1702974 (Genbank Accession No. AD000001); 1996.
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GenEmbl AD00001.
GenEmbl AD000017.
GenEmbl U00013.
GemEmbl X63508 (Nov. 20, 1996).
GenBank Z79701[gi:1524225](submitted Sep. 2, 1996; posted Sep. 6, 1996; replaced Jun. 27, 1998) (23 pages).
GenBank Z79701 Revision history (1page).
Billault Alain
Buchrieser-Brosch Roland
Cole Stewart
Gordon Stephen
Finnegan Henderson Farabow Garrett & Dunner LLP
Institut Pasteur
Sitton Jehanne
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