Method for integrating genes at specific sites in mammalian cell

Details Method for integrating genes at specific sites in mammalian cell Method for integrating genes at specific sites in mammalian cell

1998-02-13

1999-12-07

Brusca, John S.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 9141, 4353201, 435325, 435455, 435463, 536 235, 536 237, 536 2372, C12Q 168, C12N 1564, C12N 1563, C12N 510

Patent

active

05998144&

ABSTRACT:
A method for achieving site specific integration of a desired DNA at a target site in a mammalian cell via homologous recombination is described. This method provides for the reproducible selection of cell lines wherein a desired DNA is integrated at a predetermined transcriptionally active site previously marked with a marker plasmid. The method is particularly suitable for the production of mammalian cell lines which secrete mammalian proteins at high levels, in particular immunoglobulins. Novel vectors and vector combinations for use in the subject cloning method are also provided.

REFERENCES:
Thomas et al. High frequency targeting of genes to specific sited in the mammalian genome. Cell vol. 44 pp. 419-428, 1986.

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