Method for integrating genes at specific sites in mammalian cell

Details Method for integrating genes at specific sites in mammalian cell Method for integrating genes at specific sites in mammalian cell

1997-03-14

1998-11-03

Degen, Nancy

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4351723, 4353201, 536 232, C12P 2102, C12N 1563, C12N 1552

Patent

active

058306986

ABSTRACT:
A method for achieving site specific integration of a desired DNA at a target site in a mammalian cell via homologous recombination is described. This method provides for the reproducible selection of cell lines wherein a desired DNA is integrated at a predetermined transcriptionally active site previously marked with a marker plasmid. The method is particularly suitable for the production of mammalian cell lines which secrete mammalian proteins at high levels, in particular immunoglobulins. Novel vectors and vector combinations for use in the subject cloning method are also provided.

REFERENCES:
Thomas et al. High frequency targeting of genes to specific sited in the mammalian genome. Cell vol. 44 pp. 419-428, 1986.

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