Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2007-06-26
2007-06-26
Brusca, John S. (Department: 1631)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S320100
Reexamination Certificate
active
10817950
ABSTRACT:
A method for achieving site specific integration of a desired DNA at a target site in a mammalian cell via homologous recombination is described. This method provides for the reproducible selection of cell lines wherein a desired DNA is integrated at a predetermined transcriptionally active site previously marked with a marker plasmid. The method is particularly suitable for the production of mammalian cell lines which secrete mammalian proteins at high levels, in particular immunoglobulins. Novel vectors and vector combinations for use in the subject cloning method are also provided.
REFERENCES:
patent: 5202238 (1993-04-01), Fell
patent: 5464764 (1995-11-01), Capecchi
patent: 5648267 (1997-07-01), Reff
patent: 5830698 (1998-11-01), Reff et al.
patent: 5998144 (1999-12-01), Reff et al.
patent: 6413777 (2002-07-01), Reff et al.
patent: 6841383 (2005-01-01), Reff et al.
patent: WO 90/11354 (1990-10-01), None
patent: WO 91/06667 (1991-05-01), None
patent: WO 93/09222 (1993-05-01), None
patent: WO 93/24642 (1993-12-01), None
patent: WO 94/05784 (1994-03-01), None
patent: WO 94/11523 (1994-05-01), None
Sutcliffe et al., “Nucleotide sequence of the ampicillin resistance gene ofEscherichia coliplasmid pBR322,” 1978, Proc. Natl. Acad. Sci., U.S.A., 75(8): 3737-3741.
Barnett et al., “Antibody production in Chinese hamster ovary cells using an impaired selectable marker,” 1995, American Chemical Society Symposium Series, Chapter 3, 604:27-40.
al-Shawi R, et al., “Expression of a foreign gene in a line of transgenic mice is modulated by a chromosomal position effect,”Mol Cell Biol, 1990, 10: 1192-8.
Barnett RS, et al., “Antibody production in chinese hamster ovary cells using an impaired selectable marker,”ACS Symposium Series: Antibody Expression and Engineering, 1997, 604: 27-40.
Capecchi MR, “Altering the genome by homologous recombination,”Science, 1989, 244: 1288-92.
Choulika A, et al., “Induction of homologous recombination in mammalian chromosomes by using the I-Scel system ofSaccharomyces cerevisiae,” Mol Cell Biol, 1995, 15: 1968-73.
Dariavach P, et al., “Human lg superfamily CTLA-4 gene: chromosomal localization and identity of protein sequence between murine and human CTLA-4 cytoplasmic domains,”Eur J Immunol, 1988, 18: 1901-5.
Donahue TF, et al., “The nucleotide sequence of the HIS4 region of yeast,”Gene, 1982, 18: 47-59.
Flesher AR, et al., “Fluorohore-labeled carbohydrate analysis of immunoglobulin fusion proteins: Correlation of oligosachharide content with in vivo clearance profile,”Biotechnology&Bioengineering, 1995, 46: 399-407.
Fukushige S, et al., “Genomic targeting with a positive-selection lox integration vector allows highly reproducible gene expression in mammalian cells.”Proc Natl Acad Sci U S A, 1992, 89: 7905-9.
Linsley PS, et al., “CTLA-4 is a second receptor for the B cell activation antigen B7,”J Exp Med, 1991, 174: 561-9.
Meinkoth J, et al., “Unstable and stable CAD gene amplification: importance of flanking sequences and nuclear environment in gene amplification,”Mol Cell Biol, 1987, 7: 1415-24.
Morrow B, et al., “Gene targeting in mammalian cells by homologous recombination,”Curr Opin Biotechnol, 1993, 4: 577-82.
Newman R, et al., ““Primatization” of recombinant antibodies for immunotherapy of human diseases: a macaque/human chimeric antibody against human CD4.”Biotechnology(N Y), 1992, 10: 1456-60.
Peakman TC, et al., “Comparison of expression of a humanized monoclonal antibody in mouse NSO myeloma cells and Chinese hamster ovary cells,”Hum Antibodies Hybridomas. 1994, 5: 65-74.
Ramirez-Solis R et al., “Gene targeting in embryonic stem cells,”Methods in Enzymology, 225:855-878.
Reff ME, “High-level production of recombinant immunoglobulins in mammalian cells,”Curr Opin Biotechnol, 1993, 4:573-6.
Reff ME, et al., “Depletion of B cells in vivo by a chimeric mouse human monoclonal antibody to CD20,”Blood, 1994, 83: 435-45.
Rothstein R, “Targeting, disruption, replacement, and allele rescue: integrative DNA transformation in yeast,”Methods Enzymol, 1991, 194: 281-301.
Sherlock S, et al., “Delta virus hepatitis,”J Hepatol, 1986, 3: 419-23.
Sutton BJ, et al., “The human lgE network,”Nature, 1993, 366: 421-8.
Thomas KR, et al., “High frequency targeting of genes to specific sites in the mammalian genome,”Cell, 1986, 44: 419-28.
Urlaub G, et al., “Effect of gamma rays at the dihydrofolate reductase focus: deletions and inversions,”Somat Cell Mol Genet, 1986, 12:555-66.
Wahl GM, et al., “Gene amplification causes overproduction of the first three enzymes of UMP synthesis in N-(phosphonacetyl)-L-aspartate-resistant hamster cells,”J Biol Chem, 1979, 254: 8679-89.
Yoshimura FK, et al., “Different activities of viral enhancer elements before and after stable integration of transfected DNAs,”Mol Cell Biol, 1987, 7: 1296-9.
Song et al., “Accurate modification of a chomosomal plasmid by homologous recombination in human cells,” Proc. Natl. Acad. Sci. USA, p. 6820-6824, (1987).
Lin et al., “Recombination in mouse L cells between DNA introduced into cells and homologous chromosomal sequences,” Proc. Natl. Acad. Sci. USA, p. 1391-1395, (1985).
Lucas et al., “High-level production of recmobinant proteins in CHO cells using a dicistronic CHFR intron expression vector,” Nucleic Acids Research, p. 1774-1779, (1996).
Barnett Richard Spence
McLachlan Karen Retta
Reff Mitchell R.
Biogen Idec Inc.
Brusca John S.
Pillsbury Winthrop Shaw & Pittman LLP
LandOfFree
Method for integrating genes at specific sites in mammalian... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for integrating genes at specific sites in mammalian..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for integrating genes at specific sites in mammalian... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3873172