Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of...
Reexamination Certificate
2005-01-11
2005-01-11
Brusca, John S. (Department: 1631)
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
C435S320100
Reexamination Certificate
active
06841383
ABSTRACT:
A method for achieving site specific integration of a desired DNA at a target site in a mammalian cell via homologous recombination is described. This method provides for the reproducible selection of cell lines wherein a desired DNA is integrated at a predetermined transcriptionally active site previously marked with a marker plasmid. The method is particularly suitable for the production of mammalian cell lines which secrete mammalian proteins at high levels, in particular immunoglobulins. Novel vectors and vector combinations for use in the subject cloning method are also provided.
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Barnett Richard Spence
McLachlan Karen Retta
Reff Mitchell R.
Brusca John S.
Idec Pharmaceuticals Corporation
Pillsbury & Winthrop LLP
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