Chemistry: molecular biology and microbiology – Carrier-bound or immobilized enzyme or microbial cell;... – Enzyme or microbial cell is immobilized on or in an organic...
Patent
1978-03-24
1979-09-11
Shapiro, Lionel M.
Chemistry: molecular biology and microbiology
Carrier-bound or immobilized enzyme or microbial cell;...
Enzyme or microbial cell is immobilized on or in an organic...
C07G 702
Patent
active
041674472
ABSTRACT:
Insolubilized but active enzymes are prepared in a variety of ways. In one embodiment of the invention an aqueous solution of an enzyme is mixed with chitosan dissolved in water at pH 3-7 and an insolubilized product is precipitated therefrom by addition of alkali or a source of sulfate ions. Another embodiment of the invention involves contacting solid chitosan sulfate with an aqueous solution of the enzyme to be immobilized. In yet another embodiment of the invention solid chitosan is cross-linked with a polyfunctional cross-linking agent and then contacted with an aqueous solution of an enzyme. Enzymes insolubilized in accordance with the invention retain a substantial part of their original activity.
REFERENCES:
patent: 3909358 (1975-09-01), Stanley et al.
patent: 4094743 (1978-06-01), Leuba
Masri Merle S.
Randall Virginia G.
Stanley William L.
Leitereg Theodore J.
McConnell David G.
Shapiro Lionel M.
Silverstein M. Howard
The United States of America as represented by the Secretary of
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