Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
2000-05-02
2003-11-18
Yucel, Remy (Department: 1636)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
C435S006120
Reexamination Certificate
active
06649353
ABSTRACT:
I. FIELD OF THE INVENTION
The present invention relates to a method for influencing the p53 binding to target genes.
II. BACKGROUND OF THE INVENTION
p53 is a protein present in animal and human cells and is referred to as a “guardian of the genome.” p53 is a sequence-specific transactivator which is activated in the case of DNA damage. In this form, p53 binds to promoters of target genes and activates the transcription thereof. This causes growth stand-still of the cells and subsequent repair of the DNA damage and cell death, respectively.
It has turned out that p53 has lost its transactivator activity in many tumors. This is often due to the fact that the binding of p53 to the promoters of target genes is disturbed or it is not the desired target genes that are regulated.
Therefore, it is the object of the present invention to provide a product by which it is possible to influence the binding of p53 to target genes.
III. SUMMARY OF THE INVENTION
The invention relates to a method for influencing the p53 binding to a target gene, wherein the conformations of p53 and the target gene are coordinated especially by means of conformation modulators and the binding of p53 can be directly or indirectly detected.
REFERENCES:
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patent: WO 97/11367 (1997-03-01), None
patent: WO 97/14794 (1997-04-01), None
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Hansen et al., 1996, “Allosteric regulation of the thermostability and DNA binding activity of human p53 by specific interacting proteins,”J. Biol. Chem. 271(7):3917-3924.
Kim et al., 1997, “DNA-conformation is an important determinant of sequence-specific DNA binding by tumor suppressor p53,”Oncogene 15(7):857-869 (Published erratum appears inOncogene, 1997, 15(19):2385.
Deppert Wolfgang Willi
Kim Ella
Loeb Bronwen M.
Pennie & Edmonds LLP
Yucel Remy
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