Method for influencing the content of sinapine in transgenic...

Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part

Reexamination Certificate

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C800S281000, C800S285000, C800S286000, C800S287000, C800S298000, C435S320100

Reexamination Certificate

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10340811

ABSTRACT:
The invention relates to a method for influencing the sinapine content in transgenic plant cells and plants. In particular, the invention relates to the inhibition of the enzymatic activity of a UDP-glucose: sinapate glucosyltransferase (SGT) in transgenic plant cells. The invention further relates to nucleic acid molecules containing a DNA sequence which codes for a protein with the enzymatic activity of an SGT. The invention furthermore relates to transgenic plants and plant cells comprising a nucleic acid molecule according to the invention, as a result of which contain a lower content of sinapine in comparison with wild-type plants or cells, and the agricultural products and propagating material of the transgenic plants.

REFERENCES:
Milkowski C. et al. Planta 2000; vol. 211, pp. 883-886.
Lim E. et al. The Journal of Biological Chemistry, Feb. 9, 2001; vol. 276, No. 6, pp. 4344-4349.
GenBank Accession AB019232.
Database EMBL Online!; Jan. 1, 1998; Database acession No. 023401 XP002201199.
M. Milkowski; “Cloning and Heterologous Expression of a Rape cDNA Encoding UDP-glucose; sinapate glucosyltransferase”; Planta, vol. 211, 2000, pp. 883-886, XP002201197.
Database EMBL Online!; Aug. 11, 2000; Database acession No. AF287143 XP002201200.
Lim Eng-Kiat, et al.; “Identification of glucosyltransferase genes involved in sinapate metabolism and lignin synthesis in Arabidopsis”; Journal of Biological Chemistry, American Society of Biological Chemists, Baltimore, MD, US, vol. 276, No. 6, Feb. 9, 2001, pp. 4344-4349, XP0021272233; ISNN: 0021-9258.
Schawn, X., et al.; “enzymology of UDP-glucose: sinapic acid glucosyltransferasefromBrassica napus” Phytochemistry, vol. 49, No. 2, 1998, pp. 307-318, XP004290102.

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