Method for inducing the sexual maturation of lugworms

Animal husbandry – Worm culture

Reexamination Certificate

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Reexamination Certificate

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07568446

ABSTRACT:
The present invention provides a method for inducing gamete maturation in marine worms of the family arenicolidae and which exhibit epidemic spawning. In the method, male and/or female worms in a housing substrate (such as sand) in sea water are maintained at a temperature of 4 to 8° C. for 14 to 24 days, preferably 5 to 7° C. for 20 to 22 days. Spawning of the worms can them be induced either by exposure to suitable spawning promoting hormones or by raising the temperature of the sea water to 12 to 14° C.

REFERENCES:
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patent: 4417545 (1983-11-01), Finney
patent: 4513685 (1985-04-01), Frijters et al.
patent: 5664366 (1997-09-01), Lopuszanski et al.
patent: 6193902 (2001-02-01), Eguchi
patent: 6360688 (2002-03-01), Olive
patent: WO 98/06255 (1998-02-01), None
Watson et al., “Control of oocyte maturation, sperm activation and spawning in two lugworm species: Arenicola marina and A. defodiens”,Marine Ecology Progress Series175:167-176 (Dec. 17, 1998).

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