Method for inducing the acrosome reaction in human and animal sp

Details Method for inducing the acrosome reaction in human and animal sp Method for inducing the acrosome reaction in human and animal sp

1993-10-18

1995-12-12

Wityshyn, Michael G.

Chemistry: molecular biology and microbiology

Differentiated tissue or organ other than blood, per se, or...

435 4, 435 29, 436906, 436 63, A01N 102, G01N 3348

Patent

active

054748909

ABSTRACT:
A method for assessing the fertilization potential of mammalian spermatozoa comprises the steps of incubating a sperm sample with extracellular ATP, determining the level of acrosine secretion from the sperm and comparing with the level of acrosine secretion from a control sample of sperm not treated with ATP. A kit for performing the assessment of fertilization potential includes sterile culture medium, sterile, preferably lyophilized ATP, sterile culture tubes and acrosine substrate. A method of inducing the acrosome reaction in sperm taken from an infertile mammal also comprises the incubation of the sperm with extracellular ATP in appropriate culture medium. Sperm treated in this manner may be used in conventional artificial insemination and in vitro fertilization procedures.

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