Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1988-06-30
1991-09-03
Stone, Jacqueline
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
4352522, 4352523, 4353201, 435878, 536 27, 935 38, 935 64, 935 72, C12N 1509, C12N 121, C12N 111
Patent
active
050454617
ABSTRACT:
A method of increasing nodulation of a plant capable of being nodulated by Bradyrhizobium sp. (Parasponis) is provided comprising infecting said plant with a Bradyrhizobium sp. (Parasponia) species mutated such that nodK is non-functional. One such method of mutation involves insertion upstream of nodABC constitutive promoter sequences capable of activating nodABC. The invention is exemplified by the inoculation of siratro with mutated Bradyrhizobium sp. (Parasponia) mutated by insertion of nptII promoter sequences in nodK upstream of nodABC so as to activate nodABC. As compared with inoculation with the corresponding wild-type Bradyrhizobium sp. (Parasponia), nodulation onset occurred five days earlier, nodulation number was doubled, and a 120% enhancement of plant yield was shown. Similar results were obtained regardless of the orientation of the nptII promoter-containing sequences within the nodK coding sequence.
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Scott (1986) Nucl. Acids Res. 14:2905-2919.
Burn et al. (1987) Genes and Development 1:456-464.
Mazodier et al. (1985) Nucl. Acids. Res. 13:195-205.
Chereskin Che S.
Lubrizol Genetics Inc.
Stone Jacqueline
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