Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1996-02-22
1998-01-27
Prouty, Rebecca E.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435 691, 4352523, 43525233, 4353201, 536 231, 536 232, 536 237, C12N 942, C12N 120, C12P 2106, C17H 2104
Patent
active
057121427
ABSTRACT:
The gene encoding Acidothermus cellulolyticus E1 endoglucanase is cloned and expressed in Pichia pastoris. A new modified E1 endoglucanase enzyme comprising the catalytic domain of the full size E1 enzyme demonstrates enhanced thermostability and is produced by two methods. The first method of producing the new modified E1 is proteolytic cleavage to remove the cellulose binding domain and linker peptide of the full size E1. The second method of producing the new modified E1 is genetic truncation of the gene encoding the full size E1 so that the catalytic domain is expressed in the expression product.
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Mohagheghi et al., Int. J. System. Bacteriol., 36:435-443 (1986).
Lejeune et al., Biosynthesis and Biodegradation of Cellulose, C. Haigler and P.J. Weimer, Ed., Marcel-Dekker, NY 1991, pp. 623-672.
Adney William S.
Baker John O.
Chou Yat-Chen
Himmel Michael E.
Thomas Steven R.
Eure Ruth
Midwest Research Institute
O'Connor Edna M.
Prouty Rebecca E.
Richardson Ken
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