Method for increasing the growth of plant cell cultures

Details Method for increasing the growth of plant cell cultures Method for increasing the growth of plant cell cultures

1998-06-18

2000-05-30

Weber, Jon P.

Chemistry: molecular biology and microbiology

Plant cell or cell line, per se ; composition thereof;...

Culture, maintenance, or preservation techniques, per se

435431, C12N 502, C12N 500

Patent

active

060690097

ABSTRACT:
Disclosed is a method for increasing the growth rate and concentration of in vitro cultivated plant cells by re-induction and stimulation of the growth of plant cells. The method comprises the steps of: a) growing plant cell cultures in a nutrient medium under growth conditions suitable for initiation of growth; and b) supplementing cell culture with additional macronutrients between initial growing stage and before culture death in an amount sufficient to re-induce growth without being toxic to the culture. The growth is re-induced, stimulated, maintained and increased to obtain increased plant cell concentration in culture.

REFERENCES:
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Do et al., Plant Cell Tiss. Org. Cult., 27(2), "Effects of High Ammonium oncentrations on Growth and Anthocyanin Formation in Grape (Vitis-vinifera L) Cell-suspension Cultured in a Production Medium", pp. 169-174, Nov. 1991.
Mori et al., J. Food Sci., 59(3), "Production of Anthocyanin from Strawberry Cell-suspension Cultures--Effects of Sugar and Nitrogen", pp. 588-593, May 1994.
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Oksman-Caldentey et al., Plant Cell Tiss. Org. Cult., 38(2/3), "Effect of Nitrogen and Sucrose on the Primary and Secondary Metabolism of Transformed Root Cultures of Hyoscyamus-muticus", pp. 263-272, Sep. 1994.
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