Method for increasing the enzymatic reactivity of .beta.-galacto

Details Method for increasing the enzymatic reactivity of .beta.-galacto Method for increasing the enzymatic reactivity of .beta.-galacto

1990-11-05

1993-03-16

Robinson, Douglas W.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435 73, 435 74, 435 794, C12N 938

Patent

active

051943824

ABSTRACT:
In order to increase the enzymatic reactivity of .beta.-galactosidase, an azide, thiocyanate, cyanate and/or thiosulphate is added to the reaction mixture.

REFERENCES:
patent: 4891313 (1990-01-01), Berger et al.
Sigma Catalogue, 1989 p. 499.
Kirk-Ohmer, Encyclopedia of Chemical Technology, vol.s 17 & 22, pp. 611-656, 974-989 respectively, 3rd Ed., Wiley Interscience 1979.
Chebotareva et al., "Processing of Color Silver Halide Photographic Materials", Chemical Abstracts, 91:81554n, 1979.
Klose et al., "Stabilized Reagent for Detecting Hydrogen Peroxide", Chemical Abstracts 98:140126u, 1983.
Zhao et al., "Color Reaction of Zinc with Thiocyanate and Brilliant Green . . . ", Chemical Abstracts, 103:152886b, 1985.

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