Method for increasing the antibiotic-producing ability of antibi

Details Method for increasing the antibiotic-producing ability of antibi Method for increasing the antibiotic-producing ability of antibi

1995-12-21

1997-09-30

Guzo, David

Chemistry: molecular biology and microbiology

Vector, per se

536 231, 536 237, C12N 1500, C12N 1509, C07H 2104

Patent

active

056724977

ABSTRACT:
A method for increasing the antibiotic-producing ability of an antibiotic-producing microbial host cell is disclosed. The method involves transforming an antibiotic-producing microorganism with a recombinant DNA cloning vector that codes for the expression of an antibiotic biosynthetic enzyme or other gene product. The gene preferably codes for a product that is rate-limiting in the antibiotic biosynthetic pathway in Streptomyces. Plasmids that are useful for increasing tylosin production in accordance with the present method include plasmids pHJL280, pHJL284, pHJL309, pHJL311, and pHJL315. The invention further comprises microorganisms transformed with plasmids pHJL280, pHJL284, pHJL309, pHJL311, and pHJL315 and also other microorganisms and vectors used in accordance with the present method.

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