Method for increasing plasmid yield

Details Method for increasing plasmid yield Method for increasing plasmid yield

1994-03-10

1996-01-30

Fleisher, Mindy

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

43525233, C12P 1934, C12N 120

Patent

active

054879868

ABSTRACT:
This invention relates to an aqueous medium formulation which is capable of allowing the growth of a plasmid-producing host cell to a density greater than 30 at a reading of 600 nm and, in addition, enhancing plasmid production beyond that which would be expected at the cell densities achieved. The medium of the present invention comprises a carbon source in the concentration range of about 20 to about 40 grams per liter of the water component of the medium.

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patent: 5017482 (1991-05-01), Katsumata et al.
patent: 5032514 (1991-07-01), Anderson et al.
patent: 5219746 (1993-06-01), Brinegar et al.
Aaronson, CRC Handbook of Microbiology, vol. I, Laskin et al. eds., CRC Press, Cleveland, pp. 725-730.
Felgner, P. et al. (1987) "Lipofection: A highly efficient, lipid-mediated DNA-transfection procedure" Proc. Nat. Acad. Sci., USA, 84 (Nov.): 7413-7417.
Hofmann, K. H., et al. (1990) "Amplification of pBR322 Plasmid DNA in Escherichia coli relA Strains During Batch and Fed-Batch Fermentation" J. Basic Microbiol. 30(1):37-41.
Hyde, S. et al. (1993) "Correction of the ion transport defect in cystic fibrosis transgenic mice by gene therapy" Nature, 362 (Mar. 18): 250-255.

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