Method for increasing fluorescence

Details Method for increasing fluorescence Method for increasing fluorescence

1992-03-13

1994-05-31

Ceperley, Mary E.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

436501, 436531, 436537, 436538, 436546, 436827, G01N 33545

Patent

active

053169093

ABSTRACT:
The invention relates to a method based on fluorescence, especially time-resolved fluorescence for quantitative assay of a bioaffinity reaction involving bioaffinity components. The method comprises the labelling of one or several of the bioaffinity components participating in the reaction with a lanthanide chelate, forming of a lanthanide chelate for a fluorescence measurement after the reaction, and measuring the fluorescence of the chelate. The lanthanide (Eu, Tb, Sm or Dy) is brought to a strongly fluorescent form before the fluorescence measurement by incorporating the lanthanide in an aggregated particle that comprises the lanthanide chelate and a chelate of a fluorescence-increasing ion (Y, Gd, Tb, Lu or La) to bring about a cofluorescence effect. An aliphatic or aromatic beta-diketone is used as the chelating compound in the aggregate.

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