Method for inactivating dehydropepidase activity irreversibly wi

Details Method for inactivating dehydropepidase activity irreversibly wi Method for inactivating dehydropepidase activity irreversibly wi

1991-02-06

1992-05-12

Naff, David M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Enzyme inactivation by chemical treatment

435212, C12N 999, C12N 948

Patent

active

051127512

ABSTRACT:
Alkenoic acid compounds are described. The compounds which are dehydropeptidase inactivators contain a ##STR1## moiety which is substituted with a halomethylene or a cyano moiety as R.sub.3. The enzyme deprotonates the alpha-CH.sub.2 group and then the intermediate compound forms a covalent bond with enzymic residue in the active site, resulting in irreversible inactivation of the enzyme. The compounds are particularly inactivators of renal dipeptidases.

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