Method for in vivo production of a mutant library in cells

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 691, 435471, 435476, 435488, 4353201, 435489, 43525233, 435189, 435193, 435195, C12Q 168, C12N 1570, C12N 1563, C12N 910, C12N 914

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061657182

ABSTRACT:
A method for in vivo production of a library in cells comprising a multitude of mutated genetic elements, wherein an error-prone polymerase is used in each ancestral cell to replicate all or a part of a genetic element independently of the host chromosomal replication machinery. The genetic element comprises

REFERENCES:
James E. Bailey, Science, vol. 252, pp. 1668-1675, Jun. 21, 1991.

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