Method for in vitro reproduction and growth of cells in culture

Details Method for in vitro reproduction and growth of cells in culture Method for in vitro reproduction and growth of cells in culture

1989-03-10

1991-05-21

Lacey, David L.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

43524023, 435240241, 43524025, 435284, C12N 506

Patent

active

050174908

ABSTRACT:
Method and apparatus for in vitro culture of cells in culture medium to produce a viable cell mass of cell number greater than the initial cell number, wherein a cell mass of particular cell number is cultured in a first oxygen-permeable culture sub-compartment to an increased cell number, and then aseptically transferred directly from the first sub-compartment to a next serial culture sub-compartment providing additional medium and/or culture space for further growth and reproduction to produce a viable cell mass of still higher cell number. Continued transfers are made, if necessary, to successive serial culture sub-compartment until a viable cell mass of desired cell number is attained, particularly one useful for seeding of a production-scale culture system.

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Kolanko, W. et al., "Improved Yields of Monoclonal Antibodies in Flexible Plastic Containers", 1987.

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