Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide
Patent
1997-09-30
1999-04-20
Lankford, Jr., Leon B.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Enzymatic production of a protein or polypeptide
435 691, 435 701, C12N 500
Patent
active
058957537
ABSTRACT:
A method for performing coupled in vitro transcription and translation reactions is disclosed In the transcription reaction, the quantity of mRNA produced is limited to a level of less than about 2.5 micrograms in a 10 microliter volume prior to the translation elements being added. Limiting the level of mRNA produced prevents saturation of the translational processes and thus aids in the efficiency and fidelity of the translation process.
REFERENCES:
patent: 5324637 (1994-06-01), Thompson et al.
patent: 5420019 (1995-05-01), Theofan et al.
patent: 5665563 (1997-09-01), Beckler
Coen, et al., "Maize chloroplast DNA fragment encoding the large subunit of ribulosebisphosphat carboxylase," Proc. Natl. Acad. Sci. USA, 74:5487-5491 (1977).
Lewis, et al., "The Origin and Destiny of Adenovirus Proteins," Cold Spring Harbor Symposium on Quantitative Biology, vol. XXXIX pp. 581-590 ( ).
Perara & Lingappa, "A Former Amino Terminal Signal Sequence Engineered to an Internal Location Directs Translocation of Both Flanking Protein Domains," The Journal of Cell Biology, 101:2292-2301 (1985).
Stueber et al., "A novel in vitro transcription-translation system: accurate and efficient synthesis of single proteins from cloned DNA sequences," IL Press Limited 3143-3148 (1984).
Boehringer Mannheim, "Translation Kit, Reticulocyte, Type II," Cat. No. 1103 032 (1989).
Ball, et al., "Coupled Transcription and Translation in Mammalian and Avian Cell-Free Systems," Virology 84:479-495 (1978).
Bossart, et al., "Effect of UV Irradiation on the Expression of Vaccinia Virus Gene Products Synthesized in a Cell-Free System Coupling Transcription and Translation," Journal of Virology 26:673-680 (1978).
Content, et al., "Peptide Mapping Characterization of Viral Proteins Generated in a Cell-Free Coupled System for the Transcription and Translation of Influenza Virus mRNA," Journal of Virology 26:817-821 (1978).
Cooper, et al., "Transcription of Vaccinia Virus mRNA Coupled to Translation In Vitro," Virology 88:149-165 (1978).
Craig, et al., "Plasmid cDNA-directed protein synthesis in a coupled eukaryotic in vitro transcription-translation system," Nucleic Acids Research 20:4987-4995 (1992).
Dasso et al., "On the fidelity of mRNA translation in the nuclease-treated rabbit reticulocyte lysate system," Nucleic Acids Research 17:3129-3144 (1989).
Jackson, Richard J., "Potassium salts influence the fidelity of mRNA translation initiation rabbit reticulocyte lysates: unique features of encephalomyocarditis virus RNA translation," Biochimica et Biophysico Acta 1088:345-358 (1991).
Jagus,Rosemary, "Translation in Cell-Free Systems," Methods in Enzymology 152:267-276 (1987).
Kozak, Marilyn, "Context Effects and Inefficient Initiation at Non-AUG Codons in Eucaryotic Cell-Free Translation Systems," Molecular and Cellular Biology 9:5073-5080 (1989).
Krieg, et al., "Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs," Nucleic Acids Research 12:7057-7070 (1984).
Kronenberg, et al., "The 3' noncoding region of .beta.-globin mRNA is not essential for in vitro translation," Nucleic Acids Research 6:153-166 (1979).
Ledermann et al., "DNA-Directed Peptide Synthesis I. A Comparison of T.sub.2 and Escherichia coli DNA-Directed Peptide Synthesis in Two Cell-Free Systems," Biochim. Biophys. Acta 149:253-258 (1967).
McCormick et al., "S.cndot.Tag.TM.: A Multipurpose Fusion Peptide for Recombinant Proteins,".
Pelham, et al., "Characteristics of a Coupled Cell-Free Transription and Translation System Directed by Vaccinia Cores," Eut. J. Biochem. 82:199-209 (1978).
Perara, et al., "A Former Amino Terminal Signal Sequence Engineered to an Internal Location Directs Translocation of Both Flanking Protein Domains," The Journal of Cell Biology 101:2292-2301 (1985).
Roberts, et al., "Simian Virus 40 DNA Directs Synthesis of Authentic Viral Polypeptides in a Linked Transcription-Translation Cell-Free System," Proc. Nat. Acad. Sci. USA 72:1922-1926 (1975).
Rozenblatt, et al., "Direct biochemical mapping of eukaryotic viral DNA by means of a linked transcription-translation cell-free system," Proc. Natl. Acad. Sci. USA 73:2747-2751 (1976).
Sambrook, et al., "Translation of Reticulocyte Lysates," Detection and Analysis of Proteins Expressed from Cloned Genes.
Spirin, et al., "A Continuous Cell-Free Translation System of Producing Polypeptides in High Yield," Science 242:1162-1164 (1988).
Spirin, ALexander S., "Cell-Free Protein Synthesis Bioreactor," Institute of Protein Research 31-43 (1992).
Mierendorf Robert
Van Oosbree Thomas R.
Lankford , Jr. Leon B.
Novagen, Inc.
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