Method for in vitro propagation of the agent responsible for...

Details Method for in vitro propagation of the agent responsible for... Method for in vitro propagation of the agent responsible for...

2006-02-21

2006-02-21

Winkler, Ulrike (Department: 1648)

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

C424S093700, C435S363000, C435S366000

Reexamination Certificate

active

07001767

ABSTRACT:
The invention concerns a method for in vitro propagation of the agent responsible for transmissible spongiform encephalopathies, or prion, comprising steps which consist in: providing a culture or a glial cell line and in infecting the culture or line with the agent responsible for transmissible spongiform encephalopathies or prion. Said method enables to obtain infected cell line capable of being used to assess the efficacy of a molecule in the reduction or inhibition of the infectiosity of the prion or for detecting the prion.

REFERENCES:
M. Moser et al., “Developmental Expression of the Prion Protein Gene in Glial Cells,”Neuron, vol. 14, No. 3, pp. 509-517, Mar. 1995.
V. M. Roikhel et al., “Persistence of the Scrapie Agent in Glial Cells fro Rat Gasserian Ganglion,”Acta Virologica, vol. 31, No. 1, pp. 36-42, 1987.
P. Markovits et al., “Effects ofIn VitroInfection of Mouse Glial and Neuroblastoma Cells with Scrapie Agent,”Annales De Recherches Veterinaires, vol. 16, No. 1, pp. 111-119, 1985.
Butler et al., “Scrapie-Infected Murine Neuroblastoma Cells Produce Protease-Resistant Prion Proteins”, Journal of Virology, May 1988, pp 1558-1564.
Schatzl et al., “A Hypothalamic Neuronal Cell Line Persistently Infected with Scrapie Prions Exhibits Apoptosis”, Journal of Virology, Nov. 1997, pp. 8821-8831.
Fischer et al., “Prion protein (PrP) with amino-proximal deletions restoring susceptibility of PrP knockout mice to scrapie”, The EMBO Journal, vol. 15 No. 6, pp 1255-1264, 1996.
Lemaire-Vieille et al., “Epithelial and endothelial expression of the green fluorescent protein gene under the control of bovine prion protein (PrP) gene regulatory sequences in transgenic mice”, PNAS, May 9, 2000, vol. 97, No. 10, pp 5422-5427.
Brandner et al., “Normal host prion protein necessary for scrapie-induced neurotoxicity”, NATURE, VOL. 379, Jan. 25, 1996, pp 339-343.

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