Method for in vitro phosphorylation of trap of...

Details Method for in vitro phosphorylation of trap of... Method for in vitro phosphorylation of trap of...

2011-04-19

2011-04-19

Carlson, Karen Cochrane (Department: 1657)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving transferase

Reexamination Certificate

active

07927829

ABSTRACT:
The present invention relates to a method for in vitro phosphorylation of TRAP derived fromStaphylococcus aureus. In the present invention, the TRAP is first identified as a kinase that self-phosphorylates and is phosphorylated specifically in the presence of an oxidative metal ion such as iron, copper and zinc. Based upon this finding, a novel method for in vitro phosphorylation of purified TRAP is provided and also, a method for screening various chemicals and natural materials by using the above method is provided in order to select inhibitors against the TRAP phosphorylation. The inhibitors are expected to be applied for novel antibiotics ofStaphylococcus aureus, because this TRAP phosphorylation is essential to infectStaphylococcus aureus. Therefore, the present invention can be widely used to develop novel drugs againstStaphylococcus aureusand their resistant strains, in near future.

REFERENCES:
Balaban et al. “Regulation ofStaphylococcus aureuspathogenesis via target of RNAIII-activating protein (TRAP)”, JBC, 2001, 276(4):2658-2667.

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