Method for in vitro molecular evolution of protein function usin

Details Method for in vitro molecular evolution of protein function usin Method for in vitro molecular evolution of protein function usin

1998-06-16

2000-12-12

Horlick, Kenneth R.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 514 44, C12Q 168, C12P 1934

Patent

active

061596906

ABSTRACT:
The present invention relates to a method for in vitro evolution of protein function. In particular, the method relates to the shuffling of nucleotide segments obtained from exonuclease digestion. The present inventors have shown that polynucleotide fragments derived from a parent polynucleotide sequence digested with an exonuclease can be combined to generate a polynucleotide sequence which encodes for a polypeptide having desired characteristics. This method may be usefully applied to the generation of new antibodies or parts thereof having modified characteristics as compared to the parent antibody.

REFERENCES:
patent: 5605793 (1997-02-01), Stemmer
patent: 5811238 (1998-09-01), Stemmer et al.
patent: 5830721 (1998-11-01), Stemmer et al.
Sock et al., Virology 182, 298-308 (1991).
Stemmer, Nature 370, 389-391 (1994).
Stemmer, Proc. Natl. Acad. Sci. USA 91, 10747-10751 (1994).
Crameri et al., Biotechniques 18(2), 194-196 (1995).

Affiliated with

Also associated with

Rating

Method for in vitro molecular evolution of protein function usin does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for in vitro molecular evolution of protein function usin, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for in vitro molecular evolution of protein function usin will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-215359