Method for in vitro molecular evolution of protein function usin

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 912, 514 44, C12Q 168, C12P 1934

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active

061596906

ABSTRACT:
The present invention relates to a method for in vitro evolution of protein function. In particular, the method relates to the shuffling of nucleotide segments obtained from exonuclease digestion. The present inventors have shown that polynucleotide fragments derived from a parent polynucleotide sequence digested with an exonuclease can be combined to generate a polynucleotide sequence which encodes for a polypeptide having desired characteristics. This method may be usefully applied to the generation of new antibodies or parts thereof having modified characteristics as compared to the parent antibody.

REFERENCES:
patent: 5605793 (1997-02-01), Stemmer
patent: 5811238 (1998-09-01), Stemmer et al.
patent: 5830721 (1998-11-01), Stemmer et al.
Sock et al., Virology 182, 298-308 (1991).
Stemmer, Nature 370, 389-391 (1994).
Stemmer, Proc. Natl. Acad. Sci. USA 91, 10747-10751 (1994).
Crameri et al., Biotechniques 18(2), 194-196 (1995).

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