Method for in vitro molecular evolution of protein function...

Details Method for in vitro molecular evolution of protein function... Method for in vitro molecular evolution of protein function...

2006-12-26

2006-12-26

Horlick, Kenneth R. (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S091100, C435S091200

Reexamination Certificate

active

07153655

ABSTRACT:
The present invention relates to a method for in vitro evolution of protein function. In particular, the method relates to the shuffling of nucleotide segments obtained from exonuclease digestion. The present inventors have shown that polynucleotide fragments derived from a parent polynucleotide sequence digested with an exonuclease can be combined to generate a polynucleotide sequence which encodes for a polypeptide having desired characteristics. This method may be usefully applied to the generation of new proteins (e.g., antibodies and enzymes) or parts thereof having modified characteristics as compared to the parent protein.

REFERENCES:
patent: 4683202 (1987-07-01), Mullis et al.
patent: 4888286 (1989-12-01), Crea
patent: 4994368 (1991-02-01), Goodman et al.
patent: 5023171 (1991-06-01), Ho et al.
patent: 5043272 (1991-08-01), Hartley et al.
patent: 5223408 (1993-06-01), Goeddel et al.
patent: 5252479 (1993-10-01), Srivastava
patent: 5270170 (1993-12-01), Schatz et al.
patent: 5338665 (1994-08-01), Schatz et al.
patent: 5491074 (1996-02-01), Aldwin et al.
patent: 5498530 (1996-03-01), Schatz et al.
patent: 5512463 (1996-04-01), Stemmer
patent: 5514568 (1996-05-01), Stemmer
patent: 5521291 (1996-05-01), Curiel et al.
patent: 5573907 (1996-11-01), Carrino et al.
patent: 5605793 (1997-02-01), Stemmer
patent: 5712089 (1998-01-01), Borrebaeck et al.
patent: 5723323 (1998-03-01), Kauffman et al.
patent: 5733731 (1998-03-01), Schatz et al.
patent: 5733753 (1998-03-01), Jørgensen
patent: 5763192 (1998-06-01), Kauffman et al.
patent: 5798208 (1998-08-01), Crea
patent: 5811238 (1998-09-01), Stemmer et al.
patent: 5814476 (1998-09-01), Kauffman et al.
patent: 5817483 (1998-10-01), Kauffman et al.
patent: 5824514 (1998-10-01), Kauffman et al.
patent: 5830650 (1998-11-01), Crea
patent: 5830696 (1998-11-01), Short
patent: 5830721 (1998-11-01), Stemmer et al.
patent: 5834252 (1998-11-01), Stemmer et al.
patent: 5837458 (1998-11-01), Minshull et al.
patent: 5858657 (1999-01-01), Winter et al.
patent: 5858725 (1999-01-01), Crowe et al.
patent: 5871907 (1999-02-01), Winter et al.
patent: 5925544 (1999-07-01), Jørgensen
patent: 5928905 (1999-07-01), Stemmer et al.
patent: 5939250 (1999-08-01), Short
patent: 5965408 (1999-10-01), Short
patent: 5969108 (1999-10-01), McCafferty et al.
patent: 5976862 (1999-11-01), Kauffman et al.
patent: 6096548 (2000-08-01), Stemmer
patent: 6117679 (2000-09-01), Stemmer
patent: 6132970 (2000-10-01), Stemmer
patent: 6143527 (2000-11-01), Pachuk et al.
patent: 6153410 (2000-11-01), Arnold et al.
patent: 6156511 (2000-12-01), Schatz et al.
patent: 6159687 (2000-12-01), Vind
patent: 6159688 (2000-12-01), Borchert et al.
patent: 6159690 (2000-12-01), Borrebaeck et al.
patent: 6165793 (2000-12-01), Stemmer
patent: 6171820 (2001-01-01), Short
patent: 6177263 (2001-01-01), Arnold et al.
patent: 6180406 (2001-01-01), Stemmer
patent: 6238884 (2001-05-01), Short et al.
patent: 6251674 (2001-06-01), Tobin et al.
patent: 6265201 (2001-07-01), Wackett et al.
patent: 6277638 (2001-08-01), Stemmer
patent: 6287861 (2001-09-01), Stemmer et al.
patent: 6287862 (2001-09-01), delCardayre et al.
patent: 6291165 (2001-09-01), Borchert et al.
patent: 6291242 (2001-09-01), Stemmer
patent: 6291650 (2001-09-01), Winter et al.
patent: 6297053 (2001-10-01), Stemmer
patent: 6303344 (2001-10-01), Patten et al.
patent: 6309883 (2001-10-01), Minshull et al.
patent: 6319713 (2001-11-01), Patten et al.
patent: 6319714 (2001-11-01), Crameri et al.
patent: 6323030 (2001-11-01), Stemmer
patent: 6326204 (2001-12-01), delCardayre et al.
patent: 6326206 (2001-12-01), Bjornvad et al.
patent: 6329178 (2001-12-01), Patel et al.
patent: 6335160 (2002-01-01), Patten et al.
patent: 6335179 (2002-01-01), Short
patent: 6335198 (2002-01-01), delCardayre et al.
patent: 6337186 (2002-01-01), Krebber
patent: 6344356 (2002-02-01), Stemmer
patent: 6352842 (2002-03-01), Short et al.
patent: 6352859 (2002-03-01), delCardayre et al.
patent: 6355484 (2002-03-01), Patten et al.
patent: 6358709 (2002-03-01), Short et al.
patent: 6358712 (2002-03-01), Jarrell et al.
patent: 6358740 (2002-03-01), Patten et al.
patent: 6358742 (2002-03-01), Stemmer
patent: 6361974 (2002-03-01), Short et al.
patent: 6365377 (2002-04-01), Patten et al.
patent: 6365408 (2002-04-01), Stemmer
patent: 6368798 (2002-04-01), Short
patent: 6368805 (2002-04-01), Borchert et al.
patent: 6368861 (2002-04-01), Crameri et al.
patent: 6372497 (2002-04-01), Stemmer
patent: 6376246 (2002-04-01), Crameri et al.
patent: 6379964 (2002-04-01), del Cardayre et al.
patent: 6387702 (2002-05-01), Stemmer
patent: 6391552 (2002-05-01), Stemmer
patent: 6391640 (2002-05-01), Minshull et al.
patent: 6395547 (2002-05-01), Stemmer
patent: 6399383 (2002-06-01), Apt et al.
patent: 6406855 (2002-06-01), Patten et al.
patent: 6406910 (2002-06-01), Patten et al.
patent: 6413745 (2002-07-01), Patten et al.
patent: 6413774 (2002-07-01), Stemmer et al.
patent: 6420175 (2002-07-01), Stemmer
patent: 6423542 (2002-07-01), Crameri et al.
patent: 6426224 (2002-07-01), Crameri et al.
patent: 6429004 (2002-08-01), Murphy et al.
patent: 6436675 (2002-08-01), Welch et al.
patent: 6440668 (2002-08-01), Short
patent: 6444426 (2002-09-01), Short et al.
patent: 6444468 (2002-09-01), Stemmer et al.
patent: 6479258 (2002-11-01), Short
patent: 6479652 (2002-11-01), Crameri et al.
patent: 6482647 (2002-11-01), Stemmer
patent: 6483011 (2002-11-01), Stemmer et al.
patent: 6489145 (2002-12-01), Short
patent: 6492107 (2002-12-01), Kauffman et al.
patent: 6635449 (2003-10-01), Short
patent: 6709841 (2004-03-01), Short
patent: 6939689 (2005-09-01), Short et al.
patent: 6958213 (2005-10-01), Carlsson et al.
patent: 2001/0006950 (2001-07-01), Punnonen et al.
patent: 2001/0032342 (2001-10-01), Stemmer et al.
patent: 2001/0039014 (2001-11-01), Bass et al.
patent: 2001/0049104 (2001-12-01), Stemmer et al.
patent: 2001/0049125 (2001-12-01), Stemmer et al.
patent: 2002/0051976 (2002-05-01), Patten et al.
patent: 2002/0058249 (2002-05-01), Subramanian et al.
patent: 2002/0059659 (2002-05-01), Stemmer
patent: 0 368 684 (1990-05-01), None
patent: 0 415 731 (1991-03-01), None
patent: 0 456 304 (1991-11-01), None
patent: 0557897 (1993-09-01), None
patent: 0 590 689 (1994-04-01), None
patent: 2813314 (2002-03-01), None
patent: 9712512.4 (1997-06-01), None
patent: 10-66576 (1998-03-01), None
patent: 2000-308490 (2000-11-01), None
patent: 2001-57893 (2001-03-01), None
patent: 2001-197895 (2001-07-01), None
patent: WO 86/05803 (1986-10-01), None
patent: WO 90/07936 (1990-07-01), None
patent: WO 90/14430 (1990-11-01), None
patent: WO/1991/007506 (1991-05-01), None
patent: WO/1991/015581 (1991-10-01), None
patent: WO/1991/016427 (1991-10-01), None
patent: WO/1992/007075 (1991-10-01), None
patent: WO 92/01047 (1992-01-01), None
patent: WO 92/15702 (1992-09-01), None
patent: WO/1992/018645 (1992-10-01), None
patent: WO 93/07282 (1993-04-01), None
patent: WO 93/08278 (1993-04-01), None
patent: WO/1993/006213 (1993-04-01), None
patent: WO 93/12257 (1993-06-01), None
patent: WO 94/12632 (1994-06-01), None
patent: WO 94/24313 (1994-10-01), None
patent: WO 94/28173 (1994-12-01), None
patent: WO 95/22625 (1995-08-01), None
patent: WO 96/05803 (1996-02-01), None
patent: WO 96/17056 (1996-06-01), None
patent: WO 96/40750 (1996-12-01), None
patent: WO 96/40987 (1996-12-01), None
patent: WO 97/07205 (1997-02-01), None
patent: WO 97/07206 (1997-02-01), None
patent: WO 97/08320 (1997-03-01), None
patent: WO 97/20078 (1997-06-01), None
patent: WO 97/35957 (1997-10-01), None
patent: WO 97/35966 (1997-10-01), None
patent: WO 98/01581 (1998-01-01), None
patent: WO 98/05765 (1998-02-01), None
patent: WO 98/13485 (1998-04-01), None
patent: WO 98/13487 (1998-04-01), None
patent: WO 98/25965 (1998-06-01), None
patent: WO 98/27230 (1998-06-01), None
patent: WO 98/28416 (1998-07-01), None
patent: WO 98/31837 (1998-07-01), None
patent: WO 98/32845 (1998-07-01), None
patent: WO 98/41622 (1998-09-01), None
patent: WO 98/41623 (1998-09-01), None
patent: WO 98/41653 (1998-09-01), None
patent: WO 98/42832 (1998-10-01), None
patent: WO 9

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