Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2005-10-25
2005-10-25
Fredman, Jeffrey (Department: 1637)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091200, C536S024330
Reexamination Certificate
active
06958213
ABSTRACT:
The invention provides a method for generating a polynucleotide sequence or population of sequences from parent single stranded polynucleotide sequences encoding one or more protein motifs, comprising the steps of(a) providing single stranded DNA constituting plus and minus strands of parent polynucleotide sequences;(b) digesting the single stranded polynucleotide sequences with a nuclease other than DNase I to generate populations of single stranded fragments;(c) contacting said fragments generated from the plus strands with fragments generated from the minus strands and optionally, adding primer sequences that anneal to the 3′ and 5′ ends of at least one of the parent polynucleotides under annealing conditions;(d) amplifying the fragments that anneal to each other to generate at least one polynucleotide sequence encoding one or more protein motifs having altered characteristics as compared to the one or more protein motifs encoded by said parent polynucleotides.
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Barbas, III
Borrebaeck Carl
Carlsson Roland
Furebring Christina
Hager Ann-Christin Malmborg
Alligator Bioscience AB
Chunduru Suryaprabha
Dann Dorfman Herrell and Skillman
Fredman Jeffrey
Rigaut Kathleen D.
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