Method for in vitro fertilization of oocytes using microchambers

Details Method for in vitro fertilization of oocytes using microchambers Method for in vitro fertilization of oocytes using microchambers

1993-09-22

1996-04-30

Naff, David M.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

600 33, 600 34, 600 35, A61D 1900, C12N 500

Patent

active

055124763

ABSTRACT:
High efficiency in vitro fertilization is achieved when the oocytes to be fertilized are placed in individual, low volume oocyte chambers disposed about the periphery of a microdrop of fertilization medium. A sperm sample, particularly an unfractionated sperm sample, is then placed in the center of the microdrop. Motile sperm tend to move rapidly toward the periphery of the microdrop, thus resulting in an in situ separation of motile from non-motile sperm. Once at the periphery, fertilization by sperm that enter the oocyte chambers is quite facile because of the low volume of the chamber. This method of fertilization can be performed in a culture dish having a plurality of oocyte chambers formed on the interior surface of the base portion of the dish. Each oocyte chamber has a volume which exceeds the volume of an oocyte to be fertilized but is small enough to provide for facile fertilization. In general, the oocyte chamber will have a volume which exceeds the volume of the oocyte by from 800% to 2000%. The oocyte chambers are disposed about the periphery of a central circular region of the dish having a diameter of from 0.5 to 3.0 cm.

REFERENCES:
patent: 4725579 (1988-02-01), Jones, Jr. et al.
Laufer et al. Infertility, Appleton & Lange, Norwalk, CT, (1990) pp. 481-. 1
Bavister, B. D. Fertilization of Hamster Eggs In Vitro at Sperm: Egg Ratios Close to Unity, J. Exp. Zool. (1979) pp. 259-264.

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