Method for in vitro culture of mammalian cells

Details Method for in vitro culture of mammalian cells Method for in vitro culture of mammalian cells

1989-09-22

1993-06-29

Lacey, David L.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

435 701, C12N 506, C12P 2100

Patent

active

052234282

ABSTRACT:
Cells producing a desired product or products, such as antibodies, are cultured with a culture medium comprising lymph. The lymph is continuously flowed across a semi-permeable membrane, on the other side of which are the cells to be cultured. The membrane is sized so as to retain cells, and their desired products, on one side of the membrane while permitting components of the lymph-containing culture medium (flowing across the other side of the membrane) to pass through the membrane to provide nutrition, etc. to the culturing cells.

REFERENCES:
patent: 4204042 (1988-05-01), Chelle
patent: 4537860 (1985-08-01), Tolbert et al.
Rose, "Dual-Rotary Circumfusion System", Dr. Krise et al., Tissue Culture, Academic Press, 1973, 283-291.

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