Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1985-11-12
1988-05-10
Shapiro, Lionel M.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435183, 435219, 435221, 435222, 435816, C12N 952, C12N 900, C12N 950, C12N 954, C12N 956
Patent
active
047435500
ABSTRACT:
A process for the recovery of enzymes from an aqueous system having at least two aqueous phases wherein the partition coefficient with respect to an enzyme may be improved through the addition of organo-compatible, compounds having anionic functionality, such as polyacrylic acid, polyvinyl sulfonate and sodium dodecylsulfate.
REFERENCES:
patent: 3695999 (1972-10-01), Forgione
patent: 4144130 (1979-03-01), Kula et al.
patent: 4343735 (1982-08-01), Menge et al.
patent: 4451487 (1984-05-01), Vetter et al.
patent: 4572897 (1986-02-01), Amotz et al.
S. D. Flanagan, Affinity Phase Partitioning, Receptor Biochem. Methodol., 2, 15-44 (1984).
G. Johansson and M. Andersson, Parameters Determining Affinity Partitioning of Yeast Enzymes Using Polymer-Bound Trizine Dye Ligands, J. Chrom., 303, 39-51 (1984).
V. P. Shanbhag and G. Johansson, Interaction of Human Serum Albumin with Fatty Acids, Eur. J. Biochem., 93, 363-367 (1979).
G. Birkenmeier, B. Tschechonien, and G. Kopperschlager, Affinity Chromatography and Affinity Partition of Human Serum Pre-Albumin Using Immobilized Remazol Yellow GGL FEBS Letters, 174, 162-166 (1984).
C. Axelsson and V. P. Shanbhag, Histone-Hydrocarbon Interaction, Partition of Histones in Aqueous Two-Phase Systems Containing Poly(Ethylene Glycol)-Bound Hydrocarbons, Eur. J. Biochem., 71, 419-423 (1976).
G. Johansson, The effect of Poly(Ethylene Glycol) Esters on the Partition of Proteins and Fragmented Membranes in Aqueous Biphasic Systems, Bioch. Biophys. Acta, 451, 517-529 (1976).
G. Johansson and V. P. Shanbhag, Affinity Partitioning of Proteins in Aqueous Two-Phase Systems Containing Polymer-Bound Fatty Acids, J. Chrom., 284 63-72 (1984).
G. Johansson, Comparison of two Aqueous Biphasic Systems Used for the Separation of Biological Material, J. Chrom., 150, 63-71 (1978).
G. Johansson, studies on Aqueous Dextran-Poly(Ethylene Glycol) Two-Phase Systems Containing Charged Poly(Ethylene Glycol) Biochim. Biophys. Acta, 222, 381-389 (1970).
S. D. Flanagan and S. H. Barondes, A Method for Purification of Proteins Using Specific Polymer-Ligands in Aqueous Polymer Two-Phase Systems, J. Biol. Chem., 250, 1484-1489 (1975).
Ananthapadmanabhan Kavssery P.
Goddard Errol D.
Flynn Steven H.
Shapiro Lionel M.
Union Carbide Corporation
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