Method for improving non-invasive determination of the...

Surgery – Diagnostic testing – Measuring or detecting nonradioactive constituent of body...

Reexamination Certificate

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C600S322000

Reexamination Certificate

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06654620

ABSTRACT:

BACKGROUND OF THE INVENTION
1. Field of the Invention
This invention relates to a method for improving non-invasive determination of the concentration of an analyte in a human tissue, and, more particularly, a method for improving non-invasive determination of the concentration of analytes in human tissues and human body parts by applying a coupling agent at the interface between an optical measurement device and the surface of a tissue of a human.
2. Discussion of the Art
Non-invasive determination of the concentration of an analyte in a biological sample, e.g., glucose in human tissue, has been attempted by several methods. Optical methods employing infrared radiation operate on the basis that light can penetrate the tissue and then provide an absorption or scattering measurement. These methods involve the steps of introducing light and collecting light by means of optical devices having elements in contact with the skin.
Robinson et al., U.S. Pat. No. 4,975,581, describes a method for the non-invasive measurement of the concentration of glucose by detecting diffusely reflected light having a wavelength in the near infrared region of the electromagnetic spectrum. Barnes et al., U.S. Pat. No. 5,379,764, describes a method for the non-invasive measurement of the concentration of glucose via light having a wavelength in the near infrared region of the electromagnetic spectrum. The interface between the optical measurement device and the surface of the skin is formed by contacting the surface of the skin with the optical measurement device. Dahne et al., U.S. Pat. No. 4,655,225, describes an optical system for in vivo measurement of the concentration of glucose. In this system, light is transmitted from an optical element to the skin and from the skin to the optical element through the air. Caro, U.S. Pat. No. 5,348,003, describes the use of temporarily modulated electromagnetic energy for the measurement of the concentration of glucose and other analytes, but a portion of the light energy is propagated through the air to the surface of the skin and reflected back from the skin.
Marbach, “Measurement Techniques for IR Spectroscopic Blood Glucose Determination”, published in 1993, and R. Marbach, T. H. Koschinsky, F. A. Gries, and H. M. Heise, “Noninvasive Blood Glucose Assay by Near-infrared Diffuse Reflectance Spectroscopy of the Human Inner Lip”, APPLIED SPECTROSCOPY, Vol. 47, 1993, pp. 875-881, describe an optical accessory for carrying out measurements of diffuse reflectance on a human lip. That accessory suppresses the insensitivity to Fresnel or specular reflection on the skin surface area by matching the refractive index of the optical accessory to that of tissue. Calcium fluoride (CaF
2
) was disclosed as the material for constructing the optical accessory. Calcium fluoride is not an ideal index match to tissue, having an index of 1.42, relative to that of tissue, at approximately 1.38. Thus, an index mismatch occurs at the accessory to tissue interface assuming complete contact between the accessory and the tissue. The optical efficiency of the accessory is further compromised by the fact that the accessory and the tissue will not make perfect optical contact due to roughness of the surface of the tissue. The result is a significant refractive index mismatch where light is forced to travel from the accessory (refractive index=1.42) to air (refractive index=1.0) and then to tissue (refractive index=1.38). Thus, the inherent roughness of tissue results in small air gaps between the accessory and the tissue, which decrease the optical throughput of the system, and subsequently compromise the performance of the measurement accessory.
Simonsen et al., U.S. Pat. No. 5,551,422, describes a method for the determination of the scattering coefficient in tissue based on spatially resolved diffuse reflectance. A clinical apparatus and a method based on this patent employ a double-stick tape to affix the optical probe to the surface of the skin. This interface material is used for mechanical attachment purpose and does not address problems relating to measurement variations. J. T. Bruulsema, et al, “Correlation between blood glucose concentration in diabetics and noninvasively measured tissue optical scattering coefficient”, OPTICS LETTERS, Vol. 22, 1997, pp.190-192 (hereinafter “Bruulsema, et al.”), describe a clinical study based on the method of Simonsen et al., U.S. Pat. No. 5,551,422. Another clinical study was reported by L. Heinemann, et al., “Non-invasive continuous glucose monitoring in Type I diabetic patients with optical glucose sensors”, Diabetologia, Vol. 41, 1998, pp. 848-854. In both studies significant drift in the optical measurement was observed, leading to changes in the scattering coefficient independent of changes in glucose concentration and lack of correlation between changes in the scattering coefficient and changes in glucose concentration. The poor quality of the data did not allow the use of statistical analysis to correlate or predict the concentration of glucose.
The use of optical coupling agents for improving contrast and image quality in microscopic examinations is known in the art. In a classical example, immersion oil has been applied to the interface between a microscope lens and the sample object. The use of optical matching fluids to improve the precision of optical measurements is also known in the art. The use of an optical matching fluid that has the same refractive index as that of the object to be measured decreases reflection losses at the surface and improves measurement precision and accuracy.
Chance, U.S. Pat. No. 5,596,987 and Chance, U.S. Pat. No. 5,402,778, describe methods for measuring optical properties of tissue. In particular, U.S. Pat. No. 5,596,987 discloses a spectrophotometric system including a spectrophotometer with an optical input port adapted to introduce radiation into an object and an optical detection port adapted to detect radiation that has migrated through a path in the object, photon escape preventing means arranged around the object, which is relatively small, and adapted to limit escape of the introduced photons outside the object, and processing means adapted to determine an optical property of the object based on the changes between the introduced and the detected radiation. The system also includes an optical medium of a relatively large volume, forming photon escape preventing means, having selectable scattering and absorptive properties, positioning means adapted to locate the biological tissue of interest into the migration path to create a tissue-medium optical path, the optical medium substantially limiting escape of photons from the tissue-medium optical path, and processing means adapted to determine a physiological property of the tissue based on the detected optical property of the tissue-medium optical path and the scattering or absorptive properties of the optical medium. The photon escape preventing means includes an optical medium of a selectable optical property surrounding the object. The selectable optical property is an absorption or scattering coefficient. The medium has at least one optical property matched to the optical property of the object. The optical coupling system includes an optical matching fluid that is contained within a flexible, optically transparent bag and disposed partially around the monitored tissue and the excitation and detection ports of the system. The optical medium may include scattering material, such as solid particles having smooth, spherical surfaces, or styrofoam. The optical medium may include a liquid having selectable absorptive or scattering properties, such as an Intralipid solution. The optical coupling medium may include a pliable solid having selectable scattering or absorption properties. The spectrophotometric system employing such an optical medium allows one to locate tumors having optical properties different from those of normal tissue.
Messerschmidt, U.S. Pat. Nos. 5,655,530 and 5,823,951, describes an optical method for m

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