Method for importing biologically active molecules into cells

Details Method for importing biologically active molecules into cells Method for importing biologically active molecules into cells

2000-03-01

2009-08-18

Low, Christopher S. F. (Department: 1636)

Drug, bio-affecting and body treating compositions

Designated organic active ingredient containing

Peptide containing doai

C514S013800, C514S002600, C530S300000, C530S350000

Reexamination Certificate

active

07576058

ABSTRACT:
The present invention relates to the delivery of biologically active molecules, such as peptides, nucleic acids and therapeutic agents, into the interior of cells by administering to the cells a complex comprising the molecule linked to an importation competent signal peptide. Such delivery can be utilized for purposes such as peptide therapy, gene transfer, and antisense therapy.

REFERENCES:
patent: 6841535 (2005-01-01), Divita et al.
Verma et al., Gene therapy-promises, problems and prospects, 1997, Nature, vol. 389, pp. 239-242.
Luo et al., Synthetic DNA delivery systems, 2000, Nature Biotechnology, vol. 18, pp. 33-37.
Palu et al., In pursuit of new developments for gene therapy of human diseases, 1999, Journal of Biotechnology, vol. 68, pp. 1-13.
Kabouridis PS. Biological applications of protein transduction technology. Trends Biotechnol. Nov. 2003;21(11):498-503.
Lindgren et al. Cell-penetrating peptides. Trends Pharmacol Sci. Mar. 2000;21(3):99-103.
Namiki et al. Intracellular delivery of glutathione S-transferase into mammalian cells. Biochem Biophys Res Commun. Jun. 6, 2003;305(3):592-7.
Schwarze et al. Protein transduction: unrestricted delivery into all cells? Trends Cell Biol. Jul. 2000;10(7):290-5.
Veach et al. Receptor/transporter-independent targeting of functional peptides across the plasma membrane. J Biol Chem. Mar. 19, 2004:279(12):11425-31.
Redman et al. Fibrinogen biosynthesis. Assembly, intracellular degradation, and association with lipid synthesis and secretion. Ann N Y Acad Sci. 2001;936:480-95.
Agarraberes et al. Protein translocation across membranes. Biochim Biophys Acta. Jul. 2, 2001;1513(1):1-24.
Liebermeister et al. Ratcheting in post-translational protein translocation: a mathematical model. J Mol Biol. Jan. 19, 2001;305(3):643-56.
UniProtKB/Swiss-Prot database entry Q6UW56 (http://au.expasy.org/cgi-bin
iceprot.pl?APR3—HUMAN).
Jo et al. (2005) Nature Med. 11:892-898.
Jo et al. (2001) Nature Biotechnol. 19:929-933.
Product Information for ChariotTM, http://www.activemotif.com/catalog/cell—biology/chariot.
Schwarze et al., TiPS, 2000, vol. 21, pp. 45-48.
Gros et al., Biochim. Biophys. Acta, 2006, vol. 1758, pp. 384-393.
UniProtKB/Swiss-Prot database entry Q6UW56 (http://au.expasy.org/cgi-bin
iceprot.pl?APR3—HUMAN), printed from the web on Jan. 2, 2007.
Product Information for ChariotTM, http://activemotif.com/catalog/cell—biology/chariot, printed from the web on Jan. 2, 2007.
von Heijne,J. Membrane Biol. 115:195-201 (1990).
Delli Bovi et al.,Cell50:729-737 (1987).
Taira et al.,Proc. Natl. Acad. Sci. U.S.A.84:2980-2984 (1987).
Imamura et al.,Science249:1567-1570 (1990).
Imamura et al.,J. Biol. Chem. 267:5676-5679 (1992).
Baeuerle and Baltimore,Cell53:211-217 (1988).
Baeuerle and Baltimore,Science242:540-546 (1988).
Cordle et al.,J. Biol. Chem. 268:11803-11810 (1993).
Sen and Baltimore,Cell46:705-716 (1986).
Liberman and Baltimore,Mol. Cell. Biol. 10:2327-2334 (1990).
Friedman et al. Biochem. Biophys. Res. Comm. 198 (3) 1203-1208, (1994).
Lanford et al. Exp. Cell Res. 186: 32-38, (1990).
Wolff et al. Exp. Cell Res. 178: 318-334, (1988).
Delli-Bovi et al. Mol. Cell. Biol. 8 (7): 2933-2941, (1988).
Lehninger. Biochemistry. Worth Publishers. 73:75, (1975).
Kieran et al. Cell. 62: 1007-1018, (1990).
Nolan et al. Cell. 64: 961-969, (1991).
Dang et al. J. Biol. Chem. 264 (30): 18019-18023, (1989).
Lentz et al. J. Virol. 67 (3): 1414-1423 (1993).
Wessendorf et al. J. Biol. Chem. 268 (29): 22100-22104, (1993).
Stochaj et al. J. Cell Biol. 117 (3): 473-482, (1992).
Darnell et al. Molecular Cell Biol. 924-925 and 945-947 (1986).
Lin et al. J. Biol. Chem. 270(24):14255-14258 (1995).
Rojas et al. J. Biol. Chem. 271(44) 27456-27461 (1996).

Affiliated with

Also associated with

Rating

Method for importing biologically active molecules into cells does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for importing biologically active molecules into cells, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for importing biologically active molecules into cells will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-4062467