Method for importing biologically active molecules into cells

Details Method for importing biologically active molecules into cells Method for importing biologically active molecules into cells

1994-06-13

1998-09-15

Degen, Nancy

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Method of regulating cell metabolism or physiology

514 1, 514 2, 514 21, 530300, 530350, C12N 510, C07K 708, C07K 14435, A61K 3100

Patent

active

058077464

ABSTRACT:
The present invention relates to the delivery of biologically active molecules, such as peptides, nucleic acids and therapeutic agents, into the interior of cells by administering to the cells a complex comprising the molecule linked to an importation competent signal peptide. Such delivery can be utilized for purposes such as peptide therapy, gene transfer, and antisense therapy.

REFERENCES:
Friedman et al "Mutagenesis of the Nuclear Localization Sequence In fgf-1 . . . " Biochem Biophys Res. Comm. 198(3): 1203-1208 1994.
Lanford et al. "Comparison of Diverse Transport Signals . . . " Exp. Cell Res. 186: 32-38 1990.
Wolff et al. "Nuclear Protein Import . . . " Exp. Cell Res. 178 318-3341988.
Delli-Bovi et al. "Processing, Secretin & Biological Properties of a Novel Growth Factor . . . " Mol. Cell. Biol. 8(7) 2933-2941 1988.
Lehninger "Biochemistry" Worth Publishers 1975 pp. 73-75.
Kieran et al "The DNA Binding Subunit of NF-kB is Identical to Factor KBF1 . . . " Cell 62: 1007-1018 1990.
Nolan et al. "DNA Binding & 1kB Inhibition of The Cloned p. 65 Subunit of NF-kB . . . " Cell 64: 961-969 1991.
Imamura et al "Identification of a Heparin-binding Growth factor-1 Nuclear Translocation Sequence . . . " J Biol Chem. 267(8): 5676-5679 1992.
Dang et al "Nuclear & Nucleolar Targeting Sequences . . . " J. Biol. Chem. 264(30): 18019-18023 1989.
Lentz et al. "The E1 Replication Protein of Bovine Papillomvirus Type 1 Contains An Extended Nuclear Localization Signal . . . " J Virol. 67(3): 1414-1423 1993.
Wessendorf et al. "Identification of a Nuclear Localization Sequence . . . " J Biol Chem 268(29: 22100-22104 1993.
Stochaj et al. "A Conserved Phosphoprotein That Specifically Binds Nuclear localization Sequences . . . " J Cell Biol 117(3) 473-482 1992.
Darnell et al. Molecular Cell Biology pp. 924-925 & 945-947 1986.
von Heijne, J. Membrane Biol. 115:195-201 (1990).
Delli Bovi et al., Cell 50:729-737 (1987).
Taira et al., Proc. Natl. Acad. Sci. U.S.A. 84:2980-2984 (1987).
Imamura et al., Science 249:1567-1570 (1990).
Imamura et al., J. Biol. Chem. 267:5676-5679 (1992).
Baeuerle and Baltimore, Cell 53:211-217 (1988).
Baeuerle and Baltimore, Science 242:540-546 (1988).
Cordle et al., J. Biol. Chem. 268:11803-11810 (1993).
Sen and Baltimore, Cell 46:705-716 (1986).
Liberman and Baltimore, Mol. Cell. Biol. 10:2327-2334 (1990).

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