Method for immunoselection of cells using avidin and biotin

Details Method for immunoselection of cells using avidin and biotin Method for immunoselection of cells using avidin and biotin

1991-08-02

1993-06-01

Lacey, David L

Chemistry: analytical and immunological testing

Involving immune complex formed in liquid phase

Separation of immune complex from unbound antigen or antibody

424 2, 4351722, 435177, 4352401, 436501, 436530, 5303887, 53038873, C12N 1300, C12N 500, C12N 33538, A61K 3514

Patent

active

052159272

ABSTRACT:
The selectivity of immunoselection systems employing insolubilized avidin and biotinylated specific antibody is amplified, and nonspecific recovery is improved, by employing an indirect sandwich technique using a biotin-conjugated antispecies immunoglobulin that is directed to one or more nonbiotinylated specific antibodies in conjunction with insolubilized avidin. Specific cell populations can be removed and recovered from bone marrow, providing excellent recovery of bone marrow and preservation of hematopoietic stem cells for transplantation. Mixed populations of T cells or of tumor cells can be conveniently and simultaneously removed with minimal manipulation of the marrow cells. An improved positive immunoselection method provides viable and functional recovered cells, e.g., hematopoietic stem cells or activated killer cells, that can be clinically employed.

REFERENCES:
patent: 4684609 (1987-08-01), Hsu
Basch, et al., J. Immuno Methods 56:269-280 1983.

Affiliated with

Also associated with

Rating

Method for immunoselection of cells using avidin and biotin does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for immunoselection of cells using avidin and biotin, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for immunoselection of cells using avidin and biotin will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1814042