Chemistry: analytical and immunological testing – Involving immune complex formed in liquid phase – Separation of immune complex from unbound antigen or antibody
Patent
1991-10-03
1993-07-06
Lacey, David L.
Chemistry: analytical and immunological testing
Involving immune complex formed in liquid phase
Separation of immune complex from unbound antigen or antibody
435 2, 4351722, 435177, 4352401, 436501, 436530, 5303887, 53038873, C12N 1300, C12N 500, A61K 3514, G01N 33538
Patent
active
052253538
ABSTRACT:
The selectivity of immunoselection systems employing insolubilized avidin and biotinylated specific antibody is amplified, and nonspecific recovery is improved, by employing an indirect sandwich technique using a biotin-conjugated antispecies immunoglobulin that is directed to one or more nonbiotinylated specific antibodies in conjunction with insolubilized avidin. Specific cell populations can be removed and recovered from bone marrow, providing excellent recovery of bone marrow and preservation of hematopoietic stem cells for transplantation. Mixed populations of T cells or of tumor cells can be conveniently and simultaneously removed with minimal manipulation of the marrow cells. An improved positive immunoselection method provides viable and functional recovered cells, e.g., hematopoietic stem cells or activated killer cells, that can be clinically employed.
REFERENCES:
patent: 4192748 (1980-03-01), Hyden
patent: 4228237 (1980-10-01), Hevey et al.
patent: 4253996 (1981-03-01), Katz
patent: 4276206 (1981-06-01), Katz
patent: 4298685 (1981-10-01), Parikh et al.
patent: 4468470 (1984-08-01), Aalberse
patent: 4496654 (1985-01-01), Katz et al.
patent: 4512763 (1985-04-01), Schneider
patent: 4684609 (1987-08-01), Hsu
Berenson, R. J. et al., "Cellular Immunoabsorpton Using Monoclonal Antibodies," Transplantation 38(2), 136-143, 1984.
Berenson, R. J. et al., "Antigen CD34+ Marrow Cells Engraft Lethally Irradiated Baboons," J. Clin. Invest. 81, 951-955, 1988.
Lewis et al., "Separation of T and B cells using plastic surface coated with anti-immunoglobulin antibodies," Selected Methods in Cellular Immunology, Mishell et al., editors, W. H. Freeman and Co., pp. 227-234, 1980.
Edelman, "Nonenzymatic Dissociations," Tissue Culture, Kruse et al., editors, Academic Press, pp. 29-36, 1973.
Bayer, E. A. t al., "The avidin-biotin complex as a tool in molecular biology," TIBS, pp. N257-N259, Nov. 1978.
Chibata, I. and Wingard, L, B. Jr., (Eds.), "Immobilized Microbial Cells," Applied Biochemistry and Bioengineering, Wingard, L. B., Jr. et al., (Eds.), Academic Press, (vol. 4) pp. 311-349, 1983.
Berenson, R. J. et al., "Hematopoietic Stem Cell Transplants," Recent Advances in Leukemia and Lymphoma, pp. 527-523, 1987.
Berenson, R. et al., "Transplantation of CD34+ Marrow Stem Cells in Patients with Metastatic Breast Cancer," J. Cell Biochem: Abstracts, 17th Annual Meetings, abstract K401, p. 116, 1988.
Berenson, R. J., "Selective removal of T-cells and CALLA+ lymphoblasts from human bone marrow mononuclear cells using cellular immunoabsorption with monoclonal antibodies," Abstract #C-1052 for ASCO Annual Meeting, Toronto, Canada, May 6-8, 1984.
Berman, J. W. and Basch, R. S., "Amplification of Biotin-Avidin Immunofluorescence Technique," J. Immun. Methods 36, pp. 335-338, 1980.
Jasiewicz, M. L. et al., "Selective Retrieval of Biotin-Labeled Cells Using Immobilized Avidin," Exp. Cell Res. 100, 213-217, 1976.
Prud'homme, F. J. et al., "Immune dysfunction in diabetes-prone BB rats," J. Exp. Med. 159: 463-478, 1984.
Basch, R. S. et al., "Cell Separation Using Positive Immunoselective Techniques," Journal of Immunological Methods 56, pp. 269-280, 1983.
Wormmeester, J. et al., "A Simple Method for Immunoselective Cell Separation with the Avidin-Biotin System Separation with the Avidin-Biotin System," Journal of Immunological Methods 67, 389-394, 1984.
International Search Report dated May 26, 1987 for counterpart International Application No. PCT/US87/00101, filed Jan. 27, 1987.
Berenson, R. J. et al., "Positive selection of viable cell populations using avidin-biotin immunoadsorption," Journal of Immunological Methods 91, 11-19, 1986.
Hofmann, K. et al., "Avidin-biotin affinity columns: general method for attaching biotin to peptides and proteins," J. Am. Chem. Soc. 100(11), 3585-3590, 1978.
Wormmeester, J. et al., "A simple method for immunoselective cell separation with the avidin-biotin system," Biological Abstracts 78(3): No. 19564, 1984.
Berenson, R. J. et al., "Elimination of Daudi lymphoblasts from human bone marrow using avidin-biotin immunoadsorption," Blood 67(2), 509-515, 1986.
Berenson R. J. et al., "In vivo reconstitution of hematopoiesis in baboons using 12.8-positive marrow cells isolated by avidin-biotin immunoadsorption," Abstract for The American Society of Hematology 28th Annual Meeting, Dec. 6-9, 1986.
Hirn-Scavennec, J. et al., "Elimination of malignant clonogenic cells from human bone marrow using floating beads coated with monoclonal antibodies," Bone Marrow Transplantation 1 (suppl. 1), 266-267, 1986.
Ven Katasubramanian et al, "Chemical Engineering Analysis of Immobilized Cell Systems," Immobilized Microbial Cells, vol. 4, 311-349, 1983.
Vector Laboratories, Inc. Catalog pp. 1-16, 1980.
Bensinger William I.
Berenson Ronald J.
Adams Donald E.
Fred Hutchinson Cancer Research Center
Lacey David L.
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