Chemistry: analytical and immunological testing – Involving immune complex formed in liquid phase – Separation of immune complex from unbound antigen or antibody
Patent
1991-10-03
1993-11-16
Lacey, David L.
Chemistry: analytical and immunological testing
Involving immune complex formed in liquid phase
Separation of immune complex from unbound antigen or antibody
424 2, 4351722, 435177, 4352401, 436501, 436538, 5303884, 53038873, C12N 1300, C12N 500, A61K 3514, G01N 33530
Patent
active
052623343
ABSTRACT:
The selectivity of immunoselection systems employing insolubilized avidin and biotinylated specific antibody is amplified, and nonspecific recovery is improved, by employing an indirect sandwich technique using a biotin-conjugated antispecies immunoglobulin that is directed to one or more nonbiotinylated specific antibodies in conjunction with insolubilized avidin. Specific cell populations can be removed and recovered from bone marrow, providing excellent recovery of bone marrow and preservation of hematopoietic stem cells for transplantation. Mixed populations of T cells or of tumor cells can be conveniently and simultaneously removed with minimal manipulation of the marrow cells. An improved positive immunoselection method provides viable and functional recovered cells, e.g., hematopoietic stem cells or activated killer cells, that can be clinically employed.
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Bensinger William I.
Berenson Ronald J.
Adams Donald E.
Fred Hutchinson Cancer Research Center
Lacey David L.
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