Method for immunological determinations

Details Method for immunological determinations Method for immunological determinations

1974-01-28

1976-07-20

Scovronek, Joseph

Chemistry: physical processes

Physical processes

Crystallization

424 15, G01N 3106

Patent

active

039704297

ABSTRACT:
A method for immunological determinations in which use is made of a volume of a hydrophilic, insoluble, porous gel particles having binding proteins entrapped therein, and in which a fluid to be tested is introduced into the volume of gel particles whereby some of the binding sites are occupied followed by exposure of the gel particles to radio active tagged material followed by measurement of the radio activity of the unbound or bound tagged material. The binding proteins in the gel particles are returned to their original state for re-use by treatment with acidic medium to effect detachment of the bound material followed by washing to effect removal of the unbound material, and then drying.

REFERENCES:
patent: 3616936 (1971-11-01), Johansson et al.
patent: 3728290 (1973-04-01), Johansson et al.
patent: 3793445 (1974-02-01), Updike et al.
Clin. Chem., v. 19 (12), pp. 1339-1344, (1973), Updike et al.
Chem. Abstr., v. 73: 127610m (1970).
Chem. Abstr., v. 74: 123000k (1971).
Chem. Abstr., v. 75: 33018p (1971).

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