Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1989-07-07
1992-11-17
Saunders, David
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
422 56, 435 4, 435 78, 435 792, 435 793, 435805, 435970, 436 73, 436161, 436162, 436169, 436170, 436501, 436514, 436518, 436541, 436810, G01N 33543, G01N 33558
Patent
active
051642948
ABSTRACT:
Methods are disclosed for conducting assays. One such method comprises providing in combination a first bibulous member zone ("first zone") and a liquid medium containing a component. The first zone has non-diffusively bound thereto a reagent interreactive with the component. Conditions are selected wherein the liquid medium and at least a portion of the component contained therein traverse all of the first zone and migrate by capillary migration into a second bibulous member zone ("second zone"). The second zone is of a different composition than the first zone and is incapable of specifically binding the component except when an analyte is to be detected and the method further includes causing a reagent to become bound to the first bibulous member zone in relation to the amount of analyte present. The distance the component has migrated into the second zone or the difference in the distances the medium and the component have migrated into the second zone is determined, the distance or the difference being related to the amount of the component in the liquid medium or the amount of the reagent.
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WO86/04683: PCT Published International Application, Published Aug. 1986.
Ghazarossian Vartan
Shanafelt Armen B.
Skold Carl N.
Ullman Edwin F.
Leitereg Theodore J.
Saunders David
Syntex (U.S.A.) Inc.
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