Chemistry: analytical and immunological testing – Involving diffusion or migration of antigen or antibody
Patent
1994-09-01
1995-11-21
Saunders, David
Chemistry: analytical and immunological testing
Involving diffusion or migration of antigen or antibody
422 56, 422 61, 435 792, 435970, 435975, 436518, G01N 33543, G01N 33558
Patent
active
054686474
ABSTRACT:
A method is disclosed for conducting an assay for an analyte. The method comprises causing a specific binding pair member in a first aqueous medium to become bound to a first bibulous member by contacting a portion of the first bibulous member with the medium. The first bibulous member is in liquid receiving relationship with an absorbent member. The contacting is carried out under conditions wherein the first medium traverses the first bibulous member and at least a portion of the absorbent member by capillary action. The method further comprises causing the first bibulous member to come into liquid receiving relationship with a second bibulous member. A reagent in a second aqueous medium is absorbed by and preferably becomes non-diffusively bound to the second bibulous member in relation to the presence of analyte in the first medium. This is accomplished by contacting a portion of the first bibulous member with the second aqueous medium under conditions wherein the second aqueous medium traverses the first bibulous member by capillary action and at least a portion of the second bibulous member. Next, the presence of the reagent on at least a portion of the second bibulous member is determined.
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Ghazarossian Vartan
Shanafelt Armen B.
Skold Carl N.
Ullman Edwin F.
Kaku Janet K.
Leitereg Theodore J.
Saunders David
Syntex (U.S.A.) Inc.
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