Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1991-01-02
1992-11-24
Rosen, Sam
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 79, 435 792, 435 28, C12Q 128, G01N 33535
Patent
active
051660547
ABSTRACT:
A method for immoassay includes a membrane coated with a capture antibody and starch. A solution suspected of containing an antigen is passed through the membrane. Antigen binds to the capture antibody. A conjugate of a detection antibody and lactoperoxidase is passed through the membrane to form a sandwich on the membrane. A substrate composition including peroxide and iodide is passed through. The lactoperoxidase catalyzes oxidation of iodide by the peroxide to give iodine which reacts with the starch to give a blue color. The invention includes a kit of materials for performing the assay of the invention.
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Becton Dickinson and Company
Brown Richard E.
Fugit Donna R.
Rosen Sam
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