Method for immunization of mammals against atherosclerosis and p

Drug – bio-affecting and body treating compositions – Nonspecific immunoeffector – per se ; or nonspecific...

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4242831, A61K 3720, A61K 3722

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054240689

ABSTRACT:
Pharmaceutical compositions and a method for immunization of mammals against atherosclerosis including steps of a) obtaining blood from a mammal; b) isolating low density lipoprotein from the blood; c) fusing the lipoprotein in vitro to obtain heavy microemulsion lipoprotein fusion particles having new antigenic determinants; d) administering the fusion particles obtained in step (c) to a mammal; and e) allowing the immune system to clear fusion lipoprotein particles from the mammal.

REFERENCES:
patent: 4891220 (1990-01-01), Donzis
Joseph L. Witztum 1993. Role of oxidised low density lipoprotein in atherogenesis. British Heart journal No. 69 Supplement pp. S12-S18.
Fusion of Low Density Lipoproteins with Cholesteral Ester-Phospholipid Microemulsions, Parks et al. J. Biol Chem. 1985 260(5)3155-63.
Modification of Low Density Lipoproteins by Secretaoy Granules of Rat Serosal Mast Cells Kovanen and Kokkonen 1991 266(7):4430-36 J. Biol. Chem.
Technological Advances in Vaccine Development ed. Laurence Lasky .COPYRGT.1988 pp. 401-409.
"Biology of Disease--The Pathogenesis of Atherosclerosis: Atherogensis and Inflammation", by J. Michael Munro et al. Laboratory Investigation, vol. 58, No. 3, pp. 249-261, 1988.
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"Preparative and Quantitative Isolation of Plasma Lipoproteins: Rapid, Single Discontinuous Density Gradient Ultracentrifugation in a Vertical Rotor", by Byung H. Chung et al, Journal of Lipid Research, vol. 21, pp. 284-291, 1980.
"Minimally Modified Low Density Lipoprotein Stimulates Monocyte Endothelial Interactions", by Judith A. Berliner et al, J. Clin. Invest., vol. 85, pp. 1260-1266, Apr. 1990.
"Long-Term Experience with Extracorporeal Low-Density Lipoprotein Cholesterol Removal by Dextran Sulfate Cellulose Adsorption", by P. Schulzeck et al, Clin. Investig., vol. 70, pp. 99-104, 1992.
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