Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...
Patent
1994-05-17
1996-09-17
Kight, John
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
435 792, 435 10, 435 11, 435 19, 435 25, 435 28, 435805, 436 13, 436 63, 436524, 436527, 436529, 436530, C12Q 100, C12Q 128, C12Q 160, G01N 33551
Patent
active
055567436
ABSTRACT:
A dye is covalently bound to a polymeric film, especially a polyhydric polymer, for assays and other purposes. The dye may be one which, when it comes into contact with hydrogen peroxide, changes color to indicate the presence of hydrogen peroxide. This dyed film may be used in qualitative or quantitative assays. This method chemically immobilizes dyes on support matrices with much higher yields of immobilized dye than has heretofore been possible. The covalently immobilized dye may be immobilized on solid matrix particles and combined with a free-flowing dye component to form a two component dye system. By combining a dyed film-former with a film-opener, the amount of dye available for assay is greatly enhanced. This provides a dye system which can be used to detect and measure quantitatively, accurately and precisely high levels of hydrogen peroxide. These high levels of hydrogen peroxide may result from the enzyme-mediated decomposition of various analytes from undiluted whole blood samples.
REFERENCES:
patent: 3443939 (1969-05-01), Bloom et al.
patent: 3770381 (1973-11-01), Schmitt et al.
patent: 3983005 (1976-09-01), Goodhue et al.
patent: 4035146 (1977-07-01), Brenner et al.
patent: 4038031 (1977-07-01), Lam
patent: 4069016 (1978-01-01), Wu
patent: 4144306 (1979-03-01), Figueras
patent: 4247297 (1981-01-01), Berti et al.
patent: 4247642 (1981-01-01), Hirohara et al.
patent: 4251629 (1981-02-01), Yamanisi et al.
patent: 4312834 (1982-01-01), Vogel et al.
patent: 4312944 (1932-01-01), Mattiasson et al.
patent: 4394444 (1983-07-01), Cameron et al.
patent: 4435504 (1984-03-01), Zuk et al.
patent: 4533629 (1985-08-01), Litman et al.
patent: 4548905 (1985-10-01), Wu
patent: 4842976 (1989-06-01), Sanders et al.
patent: 4889636 (1989-12-01), Perry et al.
patent: 4971918 (1990-11-01), Bouse et al.
patent: 4999287 (1991-03-01), Allen et al.
patent: 5087556 (1992-02-01), Ertinghausen
Trinder. Ann. Clin. Biochem. 6:24-27, 1969.
Seitz et al. Analytical Chemistry vol. 61, No. 3: 202-205, Feb. 1989.
Zhujan et al, Poly(vinyl alcohol) as a Substrate for Indicator Immobilization for Fiber-Optic Chemical Sensors, Anal. Chem. 61, 202-205, 1989.
Gibboni David J.
Law Wai T.
ActiMed Laboratories, Inc.
Cooper Iver P.
Kight John
Kornbau Anne M.
Leary Louise N.
LandOfFree
Method for immobilizing dye on substrates does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for immobilizing dye on substrates, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for immobilizing dye on substrates will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-412376