Method for immobilizing biocatalyst in granular form with a phot

Chemistry: molecular biology and microbiology – Carrier-bound or immobilized enzyme or microbial cell;... – Enzyme or microbial cell is immobilized on or in an organic...

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435179, 435180, 435182, C12N 1110, C12N 1112, C12N 1108, C12N 1104

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active

052682862

ABSTRACT:
A biocatalyst is immobilized with a graft product of a polymer and saponified polyvinyl acetate containing a stilbazolium group as a photo-crosslinking group. The polymer is preferably gelatin, collagen, starch, cellulose, gum arabic, tragacanth gum, carrageenan, mannan, dextrin or alginic acid. The graft product is prepared by bonding the polymer to the polyvinyl acetate, either directly through functional groups of the polymer and polyvinyl acetate or through a crosslinking agent. The polymer provides affinity for the biocatalyst and by grafting the polymer to the saponified polyvinyl acetate, damage to cells upon immobilization is reduced. A liquid composition containing a biocatalyst and the graft product is added to an aqueous solution of an inorganic salt or an organic salt to form a granular gel and the gel is irradiated with actinic rays to cure the gel.

REFERENCES:
patent: 4269941 (1981-05-01), Ichimura
patent: 4791061 (1988-12-01), Sumino et al.
patent: 5051362 (1991-09-01), Suehiro et al.

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