Method for immobilization of thiol compounds via activation of p

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Albumin

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530350, 5303911, 5303913, 5303915, 5303917, 5303919, 530402, 530404, 530408, 525 61, 536 111, 536 92, 435180, C07K 1600, C07H 100, C08B 1100, C08F 800

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active

058079971

ABSTRACT:
A method for the immobilization of an organic thiol compound, HS--R, in which R is an organic residue, to a water-insoluble polymer of non-polypeptide structure exhibiting a disulfide (--S--S--) group directly bound to a saturated carbon atom at each of its sulphur atoms, which comprising contacting the polymer with an oxidation agent in such an amount and of such a kind that it is capable of transforming the disulfide group (--S--S--) to an oxidized disulfide group capable of reacting with thiol groups, whereupon the polymer obtained exhibiting one or more oxidized disulfide groups is contacted with the organic thiol compound HS--R under conditions allowing reaction to the formation of one --S--S--R group and one --SO.sub.n (H) group per disulfide oxide group that undergoes the reaction, where n is 1 or 2.

REFERENCES:
patent: 4175073 (1979-11-01), Axen et al.
patent: 4558006 (1985-12-01), Egrie
MacLaren et al.--Aust. J. Chem., 18(1965) 1655-65.
MacLaren et al--J. Soc. Dyers and Colourists, (1968-11) 564-67.
Rajca et al--Tetrahedron Letters, 31 (1990) 42 A pp. 6075-6076.

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